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We narrowed to 4,452 results for: pCMV

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Showing: 1341 - 1360 of 4452 results

  1. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid
    #160140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    160140-plasmid-map-sequence-id-317331

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  3. pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

    Plasmid
    #115136
    Purpose
    CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS
    Depositor
    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver
    Publication
    Marino et al Science. 2018 Sep 6. pii: science.aau517
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AcrVA1 anti-CRISPR protein
    Tags
    NLS(SV40)
    Expression
    Mammalian
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    115136-plasmid-map-sequence-id-220499

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  6. pCMV-dCas13-NES-eNAT10

    Plasmid
    #238299
    Purpose
    Expresses cytoplasmic version of the programmable RNA acetylation system.
    Depositor
    Won Do Heo
    Publication
    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dPspCas13b fused to eNAT10 (NAT10 Human, Prevotella sp. P5-125)
    Use
    CRISPR
    Tags
    HIV NES (C terminal of dPspCas13b)
    Expression
    Mammalian
    Mutation
    H133A for catalytically inactive mutant, deletion…
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    238299-plasmid-map-sequence-id-472414

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  7. pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

    Plasmid
    #224123
    Purpose
    Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)
    Depositor
    Brittany Adamson
    Publication
    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE7-mutant (Q20A_Y23A_Y24F_F35A)
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Q20A_Y23A_Y24F_F35A in La(SSB)1-194
    Promoter
    CMV
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    224123-plasmid-map-sequence-id-442589

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  9. pCMV-tomm20-ECFP-RspANFAST

    Plasmid
    #216348
    Purpose
    targets ECFP-RspANFAST to mitochondrial membrane
    Depositor
    Alison Tebo
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tomm20-ECFP-RspANFAST
    Tags
    tomm20
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216348-plasmid-map-sequence-id-427395

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  10. pCMV-dCas12a-ABE8e-V106W

    Plasmid
    #193646
    Purpose
    Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-TadA*8e-V106W, EGFP
    Expression
    Mammalian
    Mutation
    TadA*8e-V106W
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193646-plasmid-map-sequence-id-422057

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  11. pCMV-enCas12a-ABE8e-V106W

    Plasmid
    #193653
    Purpose
    Expression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLb-enCas12a-TadA*8e-V106W, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-D156R/G532R/K538R, TadA*8e-V106W
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193653-plasmid-map-sequence-id-422063

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  13. pCMV-MMLV-gag-hMLH1dn

    Plasmid
    #195513
    Purpose
    MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.
    Depositor
    Hyongbum Kim
    Publication
    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hMLH1dn (MLH1 Human)
    Use
    Virus like particle
    Tags
    FLAG, NES, and Protease cleavate site
    Expression
    Mammalian
    Mutation
    Human MLH1 del754-756
    Promoter
    CMV
    Available since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    195513-plasmid-map-sequence-id-384690

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  15. pCMV-MtAcKRS (human-opti)

    Plasmid
    #164195
    Purpose
    To express a Methanosarcina thermophila derived AcKRS in mammalian cells for genetic code expansion
    Depositor
    Tao Liu
    Publication
    Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MtAcKRS-478(human-opti)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    D76G; L325M; L329I; Y330F; L333A; C372F
    Promoter
    CMV
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164195-plasmid-map-sequence-id-325763

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  18. pCMV 3xFLAG-LC3B G120A

    Plasmid
    #123093
    Purpose
    Expresses 3xFLAG-LC3B G120A in mammalian cells.
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    123093-plasmid-map-sequence-id-243926

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Showing: 1341 - 1360 of 4452 results