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Showing: 1341 - 1360 of 16202 results

  1. Lazarus Gata 3 flag

    Plasmid
    #80135
    Purpose
    retroviral expression of Gata3-Flag
    Depositor
    Ellen Rothenberg
    Insert
    GATA binding protein 3 (Gata3 Mouse)
    Use
    Retroviral
    Tags
    FLAG and IRES-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    80135-plasmid-map-sequence-id-157357

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  2. 1358 pBabe puroL FKHR AAA delta DB

    Plasmid
    #10697
    Depositor
    William Sellers
    Insert
    FKHR AAA deltaDB (FOXO1 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T24A S256A S319A , deletion in aa 208-220
    Promoter
    Available since
    April 4, 2006
    Availability
    Academic Institutions and Nonprofits only

  3. pSBbi-Puro_LPAR_AKAR-mut

    Plasmid
    #125207
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted FRET-based PKA activity reporter
    Depositor
    Eric Davis
    Insert
    LPAR_AKAR-mut
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125207-plasmid-map-sequence-id-243606

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  4. pPyCAG-Nanog(L122A)-IP

    Plasmid
    #62992
    Purpose
    Expresses Mouse Nanog L122A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog L122A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Leucine 122 (123 AA in Mouse Protein) to …
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62992-plasmid-map-sequence-id-104441

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  5. pKG212

    Plasmid
    #69758
    Purpose
    Retroviral expression of CENP-T aa 1-242
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-242 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pQCXP CMV/TO RasV12 (w364-1)

    Plasmid
    #22268
    Depositor
    Eric Campeau
    Insert
    HRAS G12V (HRAS Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Glycine 12 to Valine (G12V)
    Promoter
    Available since
    Feb. 18, 2010
    Availability
    Academic Institutions and Nonprofits only
    22268-plasmid-map-sequence-id-10208

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  7. pIRESpuro3+mRFP-Utr_CH

    Plasmid
    #64358
    Purpose
    bicistronic expression of Utrophin-mRFP and Puro resistance
    Depositor
    Matthieu Piel
    Insert
    Utrophin-mRFP (UTRN Human)
    Use
    Tags
    IRES-Puro and mRFP
    Expression
    Mammalian
    Mutation
    Q236R; aa1-261 Utrophin present
    Promoter
    CMV
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    64358-plasmid-map-sequence-id-196884

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  8. pMSCV_puro_41584

    Plasmid
    #111630
    Purpose
    Complementation of Dicer_KO mESCs with wild type human DICER isoform
    Depositor
    Constance Ciaudo
    Insert
    FLAG-hsDicer (DICER1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    111630-plasmid-map-sequence-id-217596

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  9. pResQ shFEN3 3XF-FEN1 D181A

    Plasmid
    #17753
    Depositor
    Sheila Stewart
    Insert
    Flap Endonuclease I (FEN1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Triple Flag
    Expression
    Mammalian
    Mutation
    D181A
    Promoter
    Available since
    April 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  10. p663-UBC-V5-dsRed_IDG-K

    Plasmid
    #135262
    Purpose
    Gateway destination clone of dsRed (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    V5-dsRed
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135262-plasmid-map-sequence-id-268083

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  12. pPyCAG-Nanog(Y136A)-IP

    Plasmid
    #62995
    Purpose
    Expresses Mouse Nanog Y136A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y136A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 136 (137 AA in Mouse Protein) to…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62995-plasmid-map-sequence-id-104597

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  13. HA-mRIPK3 T231A/S232A-Flag/cHA-pTRIPZ vector

    Plasmid
    #73707
    Purpose
    lentiviral expression of mRIPK3 mutant
    Depositor
    Francis Chan
    Insert
    RIPK3 (Ripk3 Mouse)
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    T231A/S232A
    Promoter
    CMV/tet
    Available since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. HA-mRIPK3 AAAA-Flag/cHA-pTRIPZ vector

    Plasmid
    #73702
    Purpose
    lentiviral expression of mRIPK3 mutant
    Depositor
    Francis Chan
    Insert
    RIPK3 (Ripk3 Mouse)
    Use
    Lentiviral
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    448-AAAA-451
    Promoter
    CMV/tet
    Available since
    March 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pPyCAG-Nanog(K137A)-IP

    Plasmid
    #62996
    Purpose
    Expresses Mouse Nanog K137A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K137A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Lysine 137 (138 AA in Mouse Protein) to A…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62996-plasmid-map-sequence-id-104598

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  16. pPyCAG-Nanog(Y119A)-IP

    Plasmid
    #62991
    Purpose
    Expresses Mouse Nanog Y119A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog Y119A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Tyrosine 119 (120 AA in Mouse Protein) to…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62991-plasmid-map-sequence-id-104440

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  17. pX459_GINS4_gRNA

    Plasmid
    #232734
    Purpose
    Generates GINS4 dTAG C ternimal knock-in cell lines
    Depositor
    Junjie Chen
    Insert
    GINS4 (GINS4 Human)
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    NO
    Promoter
    Available since
    March 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    232734-plasmid-map-sequence-id-465450

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  19. pLVX ALIX-GFP S718/721A

    Plasmid
    #138583
    Purpose
    Expresses fluorescent ALIX S718/721A in mammalian cells
    Depositor
    Wesley Sundquist
    Insert
    ALIX (PDCD6IP Human)
    Use
    Lentiviral
    Tags
    GFP and h30
    Expression
    Mammalian
    Mutation
    changed serines 718 and 721 to alanines
    Promoter
    TREtight
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    138583-plasmid-map-sequence-id-322303

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  20. pAS_MmaPylT_EF1_NES-MmaPylRS WT

    Plasmid
    #174901
    Purpose
    expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Use
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174901-plasmid-map-sequence-id-348003

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Showing: 1341 - 1360 of 16202 results