We narrowed to 2,607 results for: ubiquitin

1. pET41b+_Ufd1-His

   Plasmid

   #117107

   Purpose

   Production of recombinant Ufd1-His in E.coli

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   Ufd1 (UFD1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-HA_WT POLH

   Plasmid

   #201671

   Purpose

   Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   Polymerase eta (POLH Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET41b+_Npl4

   Plasmid

   #117108

   Purpose

   Production of recombinant Npl4 in E.coli

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   Npl4 (NPLOC4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-TRIM28

   Plasmid

   #45568

   Depositor

   Fanxiu Zhu

   Article

   Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   G34S mutation in EGFP

   Promoter

   CMV

   Available since

   May 29, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. p7052 pHAGE-P-CMVt-N-HA-GAW-AURKB

   Plasmid

   #100142

   Purpose

   Expreses AURKB

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   AURKB (AURKB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQFlag-USP7 CS puroR

   Plasmid

   #46752

   Purpose

   Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C223S--catalytically inactive

   Promoter

   CMV

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-RNF2

   Plasmid

   #126544

   Purpose

   Expresses GFP-tagged RNF2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

   Insert

   RING1B (RNF2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   July 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOPINK-OTUD2 (full-length, aa 1-348)

   Plasmid

   #61409

   Purpose

   Expresses human OTUD2 (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD2 (YOD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOPINK-OTUD1 (full-length, aa 1-481)

   Plasmid

   #61405

   Purpose

   Expresses human OTUD1 (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD1 (OTUD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX6p1-GST-Rnf31

    Plasmid

    #63134

    Purpose

    Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

    Depositor

    Titia Sixma

    Article

    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

    Insert

    Rnf31 (RNF31 Synthetic, Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synthetic based on human protein sequence

    Promoter

    tac

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1-N-Myc_884-1102 USP7

    Plasmid

    #131249

    Purpose

    Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Encodes USP7 UBL domains 4-5 (USP7 amino acids 88…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. His-SUMO-hDDI2-D252N_pET11a

    Plasmid

    #169122

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D252N

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-Duet1_6xHis-TEV-eGFP-P62

    Plasmid

    #190929

    Purpose

    Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    P62 (SQSTM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5FRT/TO-Strep/HA-UBE2J1

    Plasmid

    #124669

    Purpose

    Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2J1 (UBE2J1 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOPINK-OTUD6B (full-length, aa 1-293)

    Plasmid

    #61418

    Purpose

    Expresses human OTUD6B (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3.1-Flag-H2A K13-15-118-119R

    Plasmid

    #63568

    Purpose

    Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine at 13, 15, 118, and 119 are now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-N-Myc_775-1102 USP7

    Plasmid

    #131250

    Purpose

    Mammalian expression of the USP7 UBL3-5 domains with an N-terminal Myc tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Encodes USP7 UBL domains 3-5 (USP7 amino acids 77…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOPINK-Cezanne (OTU, aa 53-446)

    Plasmid

    #61581

    Purpose

    Expresses human Cezanne (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    Cezanne (OTUD7B Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-52 and aa 447-843.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

    Plasmid

    #63565

    Purpose

    Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysines at 5, 9, 118, 119, 125, 127, and 129 are…

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pOPINK-OTUD6A (full-length, aa 1-288)

    Plasmid

    #61416

    Purpose

    Expresses human OTUD6A (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6A (OTUD6A Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge