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Plasmid#199996PurposeLanding Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donorDepositorInsertLanding Pads
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYI001
Plasmid#170833Purpose(GUSPlus::tOCS in pFP100)*DepositorInsertGUSPlus::tOCS
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG327
Plasmid#128797PurposeXylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.DepositorInsertgfpmut2 (NEWENTRY Synthetic)
UseTagsExpressionBacterialMutationPromoterPxyl(s)Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-GFP-mCherry-3pGW
Plasmid#53965PurposeBidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherryDepositorInsertsGFP
mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKL2013
Plasmid#172182PurposeA CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.DepositorInsertmCherry
UseCRISPRTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCLV3Cas-LT0
Plasmid#210548PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporterDepositorInsertCLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA
Plasmid#133339Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_ctrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKF-P14MM2AG5h
Plasmid#128254PurposeMammalian positive feedback synthetic gene circuit within a Flp-In expression vector.DepositorInsertrtTA-Advanced::P2A::EGFP
UseSynthetic Biology; Flp-in expression vectorTagsExpressionMammalianMutationPromotertight TRE promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK187
Plasmid#156423PurposeInsect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein
UseTagssfGFP-HisExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
plIVMD156
Plasmid#160934PurposeIn vivo mRNA display Gateway Destination constructDepositorInsertMCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
UseTagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastMutationPromoterMET25Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA
Plasmid#133342Purposefor constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_ctrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKDR-TetOn.1
Plasmid#183578PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)DepositorInsertAttenuator Sequence 1
UseTagsExpressionMammalianMutationPromoterTet-OnAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKDR-TetOn.4
Plasmid#183579PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)DepositorInsertAttenuator Sequence 4
UseTagsExpressionMammalianMutationPromoterTet-OnAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKDR-EF1a.4
Plasmid#183571PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)DepositorInsertAttenuator Sequence 4
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKDR-EF1a.2
Plasmid#183569PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)DepositorInsertAttenuator Sequence 2
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG217 PDLIM5 3'UTR mut
Plasmid#12047DepositorInsertN18 3'UTR mut (PDLIM5 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
SEPT5 Mut(S161A/S327A)
Plasmid#27275DepositorInsertSeptin 5 (SEPTIN5 Human)
UseTagsMyc (MASMQKLISEEDL)ExpressionMammalianMutationS161A and S327APromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1
Plasmid#133340Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentusDepositorInsertsgRNA_gcrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA
Plasmid#133334Purposefor constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_ctrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYFP-lacY-.008
Plasmid#140140PurposeEvaluating the impact of feedback and low sensor concentration on information transmission of a genetic sensor.DepositorInsertlacY (lacY )
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
SEPT5 Mut(S327A)
Plasmid#27274DepositorInsertSeptin 5 (SEPTIN5 Human)
UseTagsMyc (MASMQKLISEEDL)ExpressionMammalianMutationS327APromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
plIVMD164
Plasmid#160936PurposeIn vivo mRNA display construct for GFPDepositorInsertMCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop
UseTagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastMutationPromoterMET25Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA
Plasmid#133344Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_cpaA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG224 QKI 3'UTR mut
Plasmid#12043DepositorInsertQKI 3'UTR mut (QKI Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG216 NEK6 3'UTR mut
Plasmid#12046DepositorInsertN17 3'UTR mut (NEK6 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG178 RGS7 3'UTR mut
Plasmid#12036DepositorInsertN11 3'UTR mut (RGS7 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG226 SFRS12 3'UTR mut
Plasmid#12045DepositorInsertSFRS12 3'UTR mut (SREK1 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG220 CASC2 3'UTR mut
Plasmid#12050DepositorInsertN21 3'UTR mutant (CASC2 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG183 SRPK2 3'UTR mut
Plasmid#12041DepositorInsertN16 3'UTR mut (SRPK2 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG218 NID1 3'UTR mut
Plasmid#12048DepositorInsertN19 3'UTR mut (NID1 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAG81 ZAP3 3'UTR mut
Plasmid#12057DepositorInsertN3 3'UTR mut (YLPM1 Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLP-PH16_RS09790-pcepA-himar-chlorR
Plasmid#238494PurposeLanding Pad with BenM/PH16_RS09790, pC194+pKan+ptetQ seed promoter, pcepA-himar transposaseDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterBenM/PH16_RS09790Available sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLP-ParaBAD-pcepA-himar-chlorR
Plasmid#238491PurposeLanding Pad with AraC/ParaBAD, pC194+pKan+ptetQ seed promoter, pcepA-himar transposaseDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAraC/ParaBADAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLP-PH16_RS08125-pcepA-himar-chlorR
Plasmid#238493PurposeLanding Pad with NahR/PH16_RS08125, pC194+pKan+ptetQ seed promoter, pcepA-himar transposaseDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterNahR/PH16_RS08125Available sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHEE401_GV_MOD
Plasmid#194874PurposemCherry selection in Arabidopsis seeds in T1 and T2DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoteregg cell-specificAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ipGL2Cas
Plasmid#210555PurposeBinary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by GL2 promoter.DepositorInsertGL2p::XVE::UBQ10t::LexA::SpCas9::PEA3AT::VenusSSM
UseTagsExpressionPlantMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGE1176
Plasmid#202774PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_tNbEU
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE1177
Plasmid#202775PurposeCRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265K
Plasmid#173997PurposeGateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editingDepositorInsertUNG-rAPOBEC1(R33A)-zCas9(D10A)
UseCRISPRTags3X FLAG, NLSExpressionPlantMutationR33A in rAPOBEC1 and D10A in zCas9PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKDR-CBh.22
Plasmid#183566PurposeOne in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated CBh promoter, Fig 3)DepositorInsertAttenuator Sequence 22
UseTagsExpressionMammalianMutationPromoterCBhAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only