We narrowed to 30,758 results for: REP

1. pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115220

   Purpose

   Expresses Pin3Cas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   Pin3Cas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115210

   Purpose

   Expresses AspCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   AspCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115214

   Purpose

   Expresses PauCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   PauCas13
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA

   Plasmid

   #84474

   Purpose

   pAAV-RAM-NLS-mKate2

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   mKate2
   

   Use

   AAV

   Tags

   Nuclear localization signal of SV40 large T antig…

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA

   Plasmid

   #84468

   Purpose

   pAAV-CRAM-tdTomato

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   T230I (please see depositor comments below)

   Promoter

   TRE

   Available since

   Dec. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pC0052-REPAIR non-targeting guide clone into pC0043

   Plasmid

   #103868

   Purpose

   Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   non-targeting guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

   Plasmid

   #122084

   Purpose

   Express M.EcoGII fused to TRF1 with detabilization domain - inducible

   Depositor

   Laure Crabbe

   Article

   Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

   Insert

   DD-linker-M.EcoGII-V5-TERF1
   

   Use

   Retroviral

   Tags

   V5 tag on Nter of TERF1

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172503

   Purpose

   Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hSyn-fDIO-HA-KORD-IRES-mCitrine-WPREpA

   Plasmid

   #154871

   Purpose

   Human synapsin-1 promoter; Flp-dependent KORD-IRES-mCitrine

   Depositor

   Ulrik Gether

   Insert

   KORD-IRES-mCitrine
   

   Use

   AAV; Flp-frt

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Human Synapsin-1

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV(cmv)-NEO-FSF-tdTomato Cre reporter

    Plasmid

    #160551

    Purpose

    labeling hypoxic cells, Cre reporter

    Depositor

    Marc Vooijs

    Article

    Vermeer et al Dis Model Mech. 2020 Jul 30;13(7). pii:

    Insert

    tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115213

    Purpose

    Expresses RanCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    RanCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115218

    Purpose

    Expresses PguCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PguCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pC0051-W85X REPAIR targeting guide cloned into pC0043

    Plasmid

    #103867

    Purpose

    Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    W85X targeting guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final

    Plasmid

    #107009

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172505

    Purpose

    Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-2xFLAG-2xSTREP-CCND1(P287A)-IRES-mCherry

    Plasmid

    #172637

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(P287A) and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Pro287Ala

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid

    #109428

    Purpose

    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    mCherry T2A GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Inserted 43 base pairs into mCherry to create rep…

    Promoter

    CMV

    Available since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-2xFLAG-2xSTREP-CCND1(T286A)-IRES-mCherry

    Plasmid

    #172639

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1(T286A) and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172504

    Purpose

    Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET StrepII TEV co-transformation cloning vector (13S-R)

    Plasmid

    #48328

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge