We narrowed to 18,708 results for: met

1. p415 Glo3 R59K Δ2x+ve

   Plasmid

   #112655

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Glo3 R59K Δ2x+ve
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R59K, mutations (R223E, K224E, K225E, K233E, K234…

   Promoter

   Met25

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p415 GFP-Glo3 Δ2x+ve

   Plasmid

   #112662

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   GFP- Glo3 Δ2x+ve
   

   Use

   Tags

   sfGFP

   Expression

   Yeast

   Mutation

   Mutations (R223E, K224E, K225E, K233E, K234E, K23…

   Promoter

   Met25

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTCW018

   Plasmid

   #83531

   Purpose

   Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

   Insert

   pARO9-mf_2-pFUS1J2-ARO7i-CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pARO9/pFUS1J2

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas59

   Plasmid

   #82396

   Purpose

   sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas57

   Plasmid

   #82395

   Purpose

   sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas56

   Plasmid

   #82394

   Purpose

   sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas55

   Plasmid

   #82393

   Purpose

   sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCas52

   Plasmid

   #82392

   Purpose

   sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28 His6-mEGFP-D4H

   Plasmid

   #226373

   Purpose

   Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

   Depositor

   Catherine Tomasetto

   Article

   Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

   Insert

   D4H (pfoA Clostridium perfringens)
   

   Use

   Tags

   His6 and monomeric EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEvol-MjaYRS

    Plasmid

    #153557

    Purpose

    Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli

    Depositor

    Huiwang Ai

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038

    Inserts

    MJaYRS (first copy)
    MJaYRS (second copy)
    amber suppression tRNA under proK promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    glnS and pBAD

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-APOBEC1-YTHmut

    Plasmid

    #131637

    Purpose

    Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding

    Depositor

    Kate Meyer

    Article

    Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

    Insert

    APOBEC1-YTHmut-HA (YTHDF2 Rat, Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    YTH domain lacks AA 385-409 comprising the m6A-bi…

    Promoter

    CMV

    Available Since

    Oct. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. piggyBAC-H2B FR-MQV

    Plasmid

    #194079

    Purpose

    Expresses nuclear-localized FR-MQV in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

    Insert

    FR-MQV
    

    Use

    Tags

    Histone H2B

    Expression

    Mammalian

    Mutation

    Methionine 42 to Glutamine, Cystiene 159 to Valin…

    Promoter

    CMV7

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. NSUN2

    Plasmid

    #188059

    Purpose

    Bacterial expression plasmid for human NSUN2; for activity testing

    Depositor

    Tanja Schirmeister

    Article

    Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

    Insert

    NSUN2 (NSUN2 Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC1-SypHer3s-dmito

    Plasmid

    #108119

    Purpose

    Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s-dmito
    

    Use

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMVtight-UPRT-T2A-RFP-IRES-CD

    Plasmid

    #126677

    Purpose

    Doxycycline inducible expression of UPRT and CD along with reporter RFP

    Depositor

    Joan Massague

    Article

    Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

    Insert

    UPRT-T2A-RFP-IRES-CD (FCY1 Budding Yeast, Toxoplasma gondii)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    tight TRE

    Available Since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG94-5

    Plasmid

    #228965

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-5
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC1-SypHer3s-IMS

    Plasmid

    #108120

    Purpose

    Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s-IMS
    

    Use

    Tags

    ratiometricYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG94-15

    Plasmid

    #228971

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-15
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-pelB-LaMIgG-14

    Plasmid

    #228994

    Purpose

    For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaMIgG-14
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-EGFP-C1-HsDCP1a

    Plasmid

    #25030

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    DCP1a (DCP1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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