167,066 results

1. pCBASceI

   Plasmid

   #26477

   Purpose

   I-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI site

   Depositor

   Maria Jasin

   Article

   Richardson et al Genes Dev. 1998 Dec 15. 12(24):3831-42.

   Insert

   pCBASceI
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiGuide-Puro

   Plasmid

   #52963

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Cloning Grade DNA

   Inserts

   S. pyogenes sgRNA cassette
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1-a and hU6

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV Retrograde)

   Viral Prep

   #105553-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   Syn

   Tags

   None

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

4. M50 Super 8x TOPFlash

   Plasmid

   #12456

   Purpose

   Beta-catenin reporter. TCF/LEF sites upstream of a luciferase reporter.

   Depositor

   Randall Moon

   Article

   Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

   Insert

   TCF/LEF binding sites (Ctnnb1 )
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 13, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. OpenCRISPR-1

   Plasmid

   #221565

   Purpose

   Express OpenCRISPR-1 nuclease in human cells using a CMV promoter

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. lenti-EF1a-dCas9-VPR-Puro

   Plasmid

   #99373

   Purpose

   3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   (Sp)dCas9-VPR-P2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-S33Y Beta-catenin

   Plasmid

   #19286

   Purpose

   Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S33Y (presumptive GSK3β phosphorylation site)

   Promoter

   Available Since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-GFAP-hM3D(Gq)-mCherry (AAV5)

   Viral Prep

   #50478-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-GFAP-hM3D(Gq)-mCherry (#50478). In addition to the viral particles, you will also receive purified pAAV-GFAP-hM3D(Gq)-mCherry plasmid DNA. GFAP-driven hM4D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   GFAP

   Tags

   mCherry

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

9. mito NAD+ biosensor

   Plasmid

   #186791

   Purpose

   NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   mito NAD+ biosensor
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLIX_403

    Plasmid

    #41395

    Purpose

    Inducible lentiviral expression, TRE-gateway-V5; PGK-puro-2A-rtTA

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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