We narrowed to 2,349 results for: TIR

1. pACYCDuet-AtTIR1-Myc-MBP-HA

   Plasmid

   #222709

   Purpose

   Expression AtTIR1-Myc and MBP-HA in in bacterial cells

   Depositor

   Lei Wu

   Article

   Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

   Insert

   AtTIR1 (TIR1 Mustard Weed)
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

   Plasmid

   #217665

   Purpose

   Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   OsTir1 F74G-SNAP-Blast-AAVS1
   

   Use

   Tags

   SNAP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas9-T2A-H2B mCitirine

   Plasmid

   #210471

   Purpose

   Coexpression of Cas9 and H2B mCitrine

   Depositor

   Amro Hamdoun

   Insert

   Cas9-T2A-H2B mCitirine
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mCitrine on C terminal of H2B

   Expression

   Mammalian

   Mutation

   Cas9 contains 3xFLAG tag and nuclear localization…

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD/HisB (TIRSTD)-mEos3.2

   Plasmid

   #189719

   Purpose

   titratable expression of mEos3.2 in bacteria

   Depositor

   Daniel Daley

   Article

   Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

   Insert

   mEos3.2
   

   Use

   Tags

   His-T7-EK

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD/HisB

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. C-SWAT AID*-6MYC osTIR1

   Plasmid

   #193320

   Purpose

   C-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   AID*
   

   Use

   Tags

   6MYC

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. C-SWAT AID*-6FLAG osTIR1

   Plasmid

   #182515

   Purpose

   C-SWAT compatible vector for C-terminal tagging with AID*-6FLAG for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   AID*
   

   Use

   Tags

   6FLAG

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD/HisB (TIR23)-sfGFP

   Plasmid

   #189676

   Purpose

   titratable expression of sfGFP in bacteria

   Depositor

   Daniel Daley

   Article

   Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

   Insert

   sfGFP
   

   Use

   Tags

   His-T7-EK

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPpT4- HTA1prom-TIR1-FLAG

   Plasmid

   #189725

   Purpose

   Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   HTA1

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPpT4- TEF2prom-TIR1-FLAG

   Plasmid

   #189726

   Purpose

   Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   TEF2

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSBbi-TIR1-tTA/Zeo

    Plasmid

    #171685

    Purpose

    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin)

    Depositor

    Randy Poon

    Article

    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

    Insert

    Zeocin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RPBSA promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-CMV-TIRAP-cGASΔN-HA

    Plasmid

    #130921

    Purpose

    Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    TIRAP-ΔNcGAS
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    cGAS N terminus (aa1-159) replaced with the N ter…

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAN19-TIR1-GUS-NOSt

    Plasmid

    #108547

    Purpose

    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    GUS

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAN19-ccvTIR1-GUS-NOSt

    Plasmid

    #108548

    Purpose

    Cloning vector including C-terminally GUS-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with GUS and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    GUS

    Expression

    Mutation

    Changed F79 to G

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

    Plasmid

    #140659

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-Nluc TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAY7 (OsTIR1 mAID-EGFP-NLS TOL2)

    Plasmid

    #140533

    Purpose

    OsTIR1(WT)-P2A-mAID-EGFP-NLS TOL2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    mAID-EGFP-NLS TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

    Plasmid

    #84563

    Purpose

    Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

    Depositor

    Robert Singer

    Article

    Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

    Insert

    Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
    

    Use

    Lentiviral

    Tags

    myc tag (9x)

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

    Plasmid

    #92142

    Purpose

    Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.

    Depositor

    Benoit Bruneau

    Article

    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

    Insert

    osTir1
    

    Use

    Mouse Targeting

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

    Plasmid

    #140534

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-NLS TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

    Plasmid

    #121184

    Purpose

    OsTIR1 introduction

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    CMV-OsTIR1-loxP-PURO-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    OsTIR1 codon optimized for human cells

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

    Plasmid

    #129668

    Purpose

    A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

    Plasmid

    #121185

    Purpose

    OsTIR1 introduction

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Tet-OsTIR1-loxP-PURO-loxP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    OsTIR1 codon optimized for human cells

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

    Plasmid

    #140535

    Purpose

    OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    mAID-EGFP-NLS TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Padh1-OsTIR1(WT)-NLS-TADH1-Sp.arg3+/pUC19*

    Plasmid

    #169352

    Purpose

    Expression of OsTIR1(WT) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

    Depositor

    Tony Carr

    Insert

    OsTIR1(WT)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRS316-OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)

    Plasmid

    #198417

    Purpose

    Expresses both OsTIR1F74A and mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeast

    Depositor

    Takumi Kamura

    Article

    Ogawa et al PLoS Genet. 2023 Jun 14;19(6):e1010731.

    Insert

    OsTIR1F74A-T2A-mAID-Nb (VHHGFP4)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

    Plasmid

    #126580

    Purpose

    A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMK444 (EF1-OsTIR1(F74G) Puro)

    Plasmid

    #214374

    Purpose

    EF1-OsTIR1(F74G) (PiggyBac)

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    EF1-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTUB1:OsTIR1-3FLAG, SAG1:CAT

    Plasmid

    #87258

    Purpose

    TIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.

    Depositor

    David Sibley

    Article

    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

    Inserts

    TIR1
    CAT
    

    Use

    Toxoplasma expression

    Tags

    3FLAG

    Expression

    Mutation

    Codon optimized for Toxoplasma gondii expression

    Promoter

    TgSAG1 361 bp and TgTUB1 2717 bp

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMK395 (AAVS1 CMV-OsTIR1(F74A))

    Plasmid

    #159992

    Purpose

    Expression of OsTIR1(F74A) in human cells

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-OsTIR1(F74A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMK427 (OsTIR1(WT) mAID-EGFP-Nluc)

    Plasmid

    #140658

    Purpose

    OsTIR1(WT)-P2A-mAID-EGFP-Nluc

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(WT) mAID-EGFP-Nluc TOL2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEf1a OsTIR ires NEO pA T2BH

    Plasmid

    #127910

    Purpose

    Transposon vector containing an OsTIR IRES Neo cassette driven by the Ef1a promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    OsTIR
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge