Addgene

We narrowed to 1,257 results for: LIPI

Showing: 301 - 350 of 1257 results

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pMDC32-SEIPIN1

Plasmid

#96973

Purpose

Express Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Vinculin tail

Plasmid

#107154

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

Insert

Vinculin (VCL Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET11-His6-Pif6-sfGFP-ssrA

Plasmid

#140891

Purpose

Expression of PIF-sfGFP-ssrA in E. coli

Depositor

Neal Devaraj

Article

Bhattacharya et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

PIF-sfGFP-ssrA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PH-GAP1IP4BP

Plasmid

#20200

Depositor

Robin Irvine

Article

Hammond et al J Cell Biol. 2009 Jan 19. ():.

Insert

PH-GAP1IP4BP (RASA3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Isolated PH/Btk domain

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pBEL1324

Plasmid

#139874

Purpose

Expression plasmid for WT LetB (without TM region) for cryo and negative stain EM

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877)-6xHis

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Beclin-HA S-A

Plasmid

#46994

Purpose

Expresses Beclin1 in mammalian cells

Depositor

Kunliang Guan

Article

Russell et al Nat Cell Biol. 2013 Jul;15(7):741-50. do

Insert

becn1

Use

Tags

HA

Expression

Mammalian

Mutation

S14A

Promoter

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only
His6_BcLOV4_Q355N_mCherry_BamUK

Plasmid

#119762

Purpose

Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-Q355N_mCherry

Use

Tags

mCherry

Expression

Bacterial

Mutation

Mutated glutamine at position 355 to asparagine

Promoter

T7

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_C292A_mCherry_BamUK

Plasmid

#119761

Purpose

Encodes mCherry-tagged BcLOV4 "dark" mutant with C292A mutation for expression in bacterial cells

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-C292A_mCherry

Use

Tags

mCherry

Expression

Bacterial

Mutation

Mutated cysteine at position 292 to alanine

Promoter

T7

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA PTEN KD2

Plasmid

#162009

Purpose

PTEN shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PTEN (PTEN Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMDC32-SEIPIN3

Plasmid

#96975

Purpose

Express Arabidopsis SEIPIN3 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN3 (AT2G34380 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2003

Plasmid

#139888

Purpose

Expression plasmid for LetAB with 5 ring (rings 12347) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta515-746)-6xHis

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2000

Plasmid

#139885

Purpose

Expression plasmid for LetAB with 2 ring (rings17) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta161-747)-6xHis

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-Scs2

Plasmid

#58834

Purpose

Expresses GFP-Tagged Scs2 in yeast

Depositor

Tim Levine

Article

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Scs2 (SCS2 Budding Yeast)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-GAP1IP4BPdeltaC2

Plasmid

#20199

Depositor

Robin Irvine

Article

Hammond et al J Cell Biol. 2009 Jan 19. ():.

Insert

GAP1IP4BP-detlaC2 (RASA3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deletion of C2 domains

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-TCF7L2

Plasmid

#232644

Purpose

transient expresses TCF7L2 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

TCF7L2 (TCF7L2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

Plasmid

#222988

Purpose

Bacterial expression of iGeoCas9(C2) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TMEM63A-peGFP-N1

Plasmid

#228590

Purpose

Expresses mouse TMEM63A-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Plasmid

#222987

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGW1-ExSYTE-P2A-EGFP

Plasmid

#216257

Purpose

ExSYTE (P2A) EGFP

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE P2A EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGW1-ExSYTE-EGFP

Plasmid

#216259

Purpose

ExSYTE - EGFP fusion

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-HE-ExSYTE

Plasmid

#216265

Purpose

ExSYTE in oocyte vector

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE

Use

Crna synthesis for xenopus laevis oocyte

Tags

none

Expression

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pJPT001-HaloTag-1x FLAG

Plasmid

#202492

Purpose

Express "soluble" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Use

Tags

1x FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJPT004-1x FLAG-HaloTag-G

Plasmid

#202495

Purpose

Express "G" HaloTag

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

HaloTag

Use

Tags

1x FLAG and Geranylgeranyl site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2403

Plasmid

#195642

Purpose

Complementation of MlaC(WT)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2005

Plasmid

#139880

Purpose

Complementation plasmid for LetAB with 2 ring (rings17) LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(delta161-747)

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2004

Plasmid

#139889

Purpose

Expression plasmid for LetAB with 6 ring (rings 123457) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta634-746)-6xHis

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1993

Plasmid

#139897

Purpose

Expression plasmid for LetB PLL1 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta114-128, P113G)-6xHis

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1999

Plasmid

#139903

Purpose

Expression plasmid for LetB PLL7 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta816-826, S815G, T827G)-6xHis

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRNA2

Plasmid

#174005

Purpose

Used to generate stable mRNA for heterologous expression experiments (no polyA)

Depositor

Stephen Ikeda

Article

Williams et al Front Neurosci. 2010 Nov 4;4:181. doi: 1

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
WIPI2d-TEVcs-TSF

Plasmid

#171419

Purpose

For the purifcation of WIPI2d protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

WIPI2d (WIPI2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2298

Plasmid

#164603

Purpose

Expression and Complementation plasmid for MlaD (A17F, A20F, V24F)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Use

Tags

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Ala17Phe, Ala20Phe, Val24P…

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 Puro shRNA PIK3C2G KD1

Plasmid

#162008

Purpose

PIK3C2gamma shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2G (PIK3C2G Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 Puro shRNA SNX9 KD1

Plasmid

#162014

Purpose

SNX9 shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

SNX9 (SNX9 Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 Puro shRNA SNX9 KD2

Plasmid

#162015

Purpose

SNX9 shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

SNX9 (SNX9 Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 Puro shRNA PIK3C2A KD5

Plasmid

#162006

Purpose

PIK3C2alpha shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2A (PIK3C2A Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1 Puro shRNA PTEN KD4

Plasmid

#162010

Purpose

PTEN shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PTEN (PTEN Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1996

Plasmid

#139900

Purpose

Expression plasmid for LetB PLL4 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta459-360)-6xHis

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2042

Plasmid

#139927

Purpose

Complementation plasmid for LetAB with 4 ring (rings 1347) LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB (1-159; 280-515; 747-877)

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2041

Plasmid

#139926

Purpose

Complementation plasmid for LetAB with 4 ring (rings 1237) LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB (1-391; 747-877)

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1892

Plasmid

#139896

Purpose

Complementation plasmid for LetAB with LetB PLL7 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(delta816-826, S815G, T827G)

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2040

Plasmid

#139930

Purpose

Complementation plasmid for LetAB with LetB TM region replaced by PelB leader sequence

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-(PelB-LetB(43-877))-6xHis

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1890

Plasmid

#139894

Purpose

Complementation plasmid for LetAB with LetB PLL5 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(delta581-590, K580G, Q591G)

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2045

Plasmid

#139924

Purpose

Expression plasmid for 4 ring (rings 1347) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB (43-159; 280-515; 747-877)

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2044

Plasmid

#139923

Purpose

Expression plasmid for 4 ring (rings 1237) LetB (without TM region) mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB (43-391; 747-877)

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1998

Plasmid

#139902

Purpose

Expression plasmid for LetB PLL6 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta703-708, Q702G, E709G)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1997

Plasmid

#139901

Purpose

Expression plasmid for LetB PLL5 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta581-590, K580G, Q591G)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1995

Plasmid

#139899

Purpose

Expression plasmid for LetB PLL3 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta347-360, P346G)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1891

Plasmid

#139895

Purpose

Complementation plasmid for LetAB with LetB PLL6 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(delta703-708, Q702G, E709G)

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1887

Plasmid

#139891

Purpose

Complementation plasmid for LetAB with LetB PLL2 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(delta229-239, D228G, L240G)

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1888

Plasmid

#139892

Purpose

Complementation plasmid for LetAB with LetB PLL3 deletion

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(delta347-360, P346G)

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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