1. p426 103Q GPD

   Plasmid

   #1184

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pcDNA3-blueCKAR

   Plasmid

   #118472

   Purpose

   cpBFP-based single-color biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   blueCKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDRF1-GW ymScarletI

   Plasmid

   #118452

   Purpose

   Overexpression of yeast codon-optimized mScarletI

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymScarletI
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDRF1-GW ytdTomato

   Plasmid

   #118456

   Purpose

   Overexpression of yeast codon-optimized tdTomato

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ytdTomato
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1(+)-ExRai-AKAR

   Plasmid

   #118405

   Purpose

   cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   ExRai-AKAR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDRF1-GW ymVenus

   Plasmid

   #118454

   Purpose

   Overexpression of yeast codon-optimized mVenus

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymVenus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDRF1-GW ymTurquoise2

   Plasmid

   #118453

   Purpose

   Overexpression of yeast codon-optimized mTq2

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-SpCas9-2A-GFP-noITR

   Plasmid

   #118415

   Purpose

   Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDRF1-GW ymYPET

   Plasmid

   #118455

   Purpose

   Overexpression of ymYPET

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymYPET
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   YPET A206K, F208S, E232L, N235D

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEmCherry2

   Plasmid

   #118413

   Purpose

   Red fluorescent reporter for assessing CRISPR gRNA activity

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDRF1-GW yomCherry-T2A-mTurquoise2

    Plasmid

    #118447

    Purpose

    Produces equimolar expression of yeast codon-optimized mCherry and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yomCherry-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMRE141

    Plasmid

    #118493

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1(+)-PKI-GFP

    Plasmid

    #118482

    Purpose

    GFP-tagged Protein Kinase A inhibitor peptide.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    PKI-GFP
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-ExRai-AktAR(T/A)

    Plasmid

    #118463

    Purpose

    Negative-control mutant for ExRai-AktAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AktAR(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-RAB-EKARev(T/A)

    Plasmid

    #118475

    Purpose

    Negative-control mutant for RAB-EKARev biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev(T/A)
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)-blueAKAR

    Plasmid

    #118470

    Purpose

    cpBFP-based single-color biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueAKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-Lyn-RAB-EKARev

    Plasmid

    #118477

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    Lyn-RAB-EKARev
    

    Use

    Tags

    N-terminal targeting sequence from Lyn-kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-ymTurquoise2-CaURA3

    Plasmid

    #118458

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selection

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-ExRai-CKAR(T/A)

    Plasmid

    #118410

    Purpose

    Negative-control mutant for ExRai-CKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-CKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMRE146

    Plasmid

    #118498

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge