We narrowed to 12,552 results for: NUC

1. pCAG-nucGFP11-3xPax7gRNA

   Plasmid

   #224570

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Nuc-deltaCKAR

   Plasmid

   #222430

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Nucleus delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEntry_HCoV-HKU1-Nucleocapsid2

   Plasmid

   #168907

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Pascal Falter-Braun

   Article

   Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

   Insert

   HCoV-HKU1-Nucleocapsid2 (N2 )
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-29b(+)_NSP4ss_SerM_NucA-His

   Plasmid

   #218190

   Purpose

   Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

   Depositor

   Florence Pojer

   Insert

   nucA
   

   Use

   Tags

   His and NSP4 signal secretion peptide

   Expression

   Bacterial

   Mutation

   codon optimized ecoli residues - 22-266

   Promoter

   T7

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICH-nuc-roGFP2-synORP1

   Plasmid

   #191759

   Purpose

   Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   nuc-roGFP2-synORP1
   

   Use

   Binary agrobacterium ti plasmid

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(Hygro) TagBFP-hNucleolin

   Plasmid

   #182592

   Purpose

   expresses bfp-hNucleolin in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hNucleolin (NCL Human)
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD454-SR mouse Alpha-synuclein

   Plasmid

   #89075

   Purpose

   bacterial expression of mouse Alpha-synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   Alpha-synuclein (Snca Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 17, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET-alpha-synuclein-BiFC

   Plasmid

   #87855

   Purpose

   pETDUET-1 based vector for BiMolecular Fluorescence Complementation based assays of alpha-synuclein oligomerisation

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Inserts

   alpha-synuclein-VC155
   alpha-synuclein-VN154
   

   Use

   Tags

   C-terminal fragment of mVenus BiFC protein and N-…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28C-SARS-CoV-2-Nucleocapsid

   Plasmid

   #204402

   Purpose

   E. coli expression of full-length SARS-CoV-2 nucleocapsid

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   SARS-CoV-2 nucleocapsid (N SARS-CoV-2)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Nuc-ExRai-AktAR2

    Plasmid

    #184049

    Purpose

    Nuclear-targeted ExRai-AktAR2.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Nuc-ExRai-AktAR2
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR4-HA-myc-nuc (w557-1)

    Plasmid

    #20025

    Purpose

    Gateway entry vector for nuclear targeting of domains (N-term HA , C-term NLS, myc tags)

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    HA, Myc, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV/myc/Nuc-hOGG1

    Plasmid

    #18709

    Depositor

    David Sidransky

    Article

    Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

    Insert

    8-oxoguanine DNA glycosylase (OGG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pENTR4-myc-nuc (w556-1)

    Plasmid

    #20026

    Purpose

    Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Gateway entry vector

    Tags

    Myc and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. nuclear EKAR (EGFP-mRFP)

    Plasmid

    #18682

    Depositor

    Karel Svoboda

    Article

    Harvey et al PNAS 2008 Dec 9;105(49):19264-9

    Insert

    ERK Activity Reporter
    

    Use

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOTTC293 - pAAV EF1a V5-synuclein (WT)

    Plasmid

    #60057

    Purpose

    An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    alpha-synuclein (SNCA Human, human)
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. nuclear EKAR (Cerulean-Venus)

    Plasmid

    #18681

    Depositor

    Karel Svoboda

    Article

    Harvey et al PNAS 2008 Dec 9;105(49):19264-9

    Insert

    ERK Activity Reporter
    

    Use

    Tags

    monomeric Cerulean and monomeric Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG426-GAL-Alpha-synuclein

    Plasmid

    #181715

    Purpose

    Galactose inducible expression of Alpha-synuclein

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOpen-Exonuclease III E. coli

    Plasmid

    #165571

    Purpose

    Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    Exonuclease III E. coli
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pD454-SR rat Alpha-synuclein

    Plasmid

    #89074

    Purpose

    bacterial expression of rat Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCDNA3.0_nucLAMA-G97 IRES EGFP

    Plasmid

    #136620

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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