We narrowed to 432 results for: Pef1

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pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN

Plasmid

#69582

Purpose

Expresses p53 tagged with mKate2 and split N-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - N term

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1a-myc-LRH1(ES)-IRES-neo

Plasmid

#28092

Depositor

Austin Cooney

Article

Wagner et al Stem Cells. 2010 Oct . 28(10):1794-804.

Insert

Lrh-1 (Nr5a2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
pEF1α-squirrel-monkey-CHMP3(155)-HA

Plasmid

#154173

Purpose

expresses squirrel monkey CHMP3(155) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3(155)

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 155

Promoter

EF1-alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-owl-monkey-retroCHMP3(148)-HA

Plasmid

#154252

Purpose

expresses prematurely truncated owl monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of retroCHMP3 at amino acid…

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN

Plasmid

#69586

Purpose

Expresses mutated p53-L344P tagged with mKate2 and split N-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53-L344P (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - N term

Expression

Mammalian

Mutation

p53 L344P mutation that prevents dimerization and…

Promoter

EF1alpha

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1-a_LbuCas13a-HA-IRES-BFP-2A-BSD

Plasmid

#251219

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven LbuCas13a expressing cells.

Depositor

Niels Geijsen

Insert

LbuCas13a

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-FRT-HaloTag-RPA194-E593Q

Plasmid

#247349

Purpose

Expresses HaloTag-RPA194-E593Q

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A) (POLR1A Human)

Tags

HaloTag

Expression

Mammalian

Mutation

RPA194 (POLR1A), mutated with E593Q

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pEf1a-Dn29-dCas9-P2A-GFP

Plasmid

#247155

Purpose

Mammalian expression of a human codon optimized wildtype Dn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-dCas9-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1α-woolly-monkey-retroCHMP3(148)-HA

Plasmid

#154250

Purpose

expresses prematurely truncated woolly monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of retroCHMP3 at amino acid…

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1α-pygmy-marmoset-retroCHMP3(148)-HA

Plasmid

#154251

Purpose

expresses prematurely truncated pygmy marmoset retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of retroCHMP3 at amino acid…

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ashL344A-mKate2-splitmVenusN

Plasmid

#69584

Purpose

Expresses mutated p53-L344A tagged with mKate2 and split N-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53-L344A (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - N term

Expression

Mammalian

Mutation

p53 L344A mutation that prevents tetramerization …

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

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pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC

Plasmid

#69585

Purpose

Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53-L344A (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - C term

Expression

Mammalian

Mutation

p53 L344A mutation that prevents tetramerization …

Promoter

EF1alpha

Availability

Academic Institutions and Nonprofits only

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AAV pEF1a-DIO-FLPo-WPRE-hGHpA

Plasmid

#87306

Purpose

An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

Depositor

Li Zhang

Article

Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

Cre-dependent mouse codon-optimized Flp recombinase.

Use

AAV

Tags

N/A

Expression

Mammalian

Promoter

EF1a

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI

Plasmid

#202596

Purpose

plasmid for expressing the DNMT3bCI (catalytic inactive)

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

H2B-mCherry-T2A-rTetR-DNMT3bCI (Dnmt3b Synthetic, Mouse)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Mutation

P656V, C657D

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

Plasmid

#197687

Purpose

plasmid for expressing the DNMT3bCD

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

Plasmid

#206213

Purpose

PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

Depositor

Masamichi Kamihira

Article

Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

Insert

tetracycline-responsive transactivator

Expression

Mammalian

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

Plasmid

#100944

Purpose

Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Human, Synthetic)

Tags

3xTy1

Expression

Mammalian

Mutation

F636A, E660A, E725A, R295A

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE

Plasmid

#129704

Purpose

Express HA-KASH for nuclei labeling and purification

Depositor

Yi Zhang

Article

Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

Insert

HA-VHH-KASH

Use

AAV and Cre/Lox

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTRE-SOX17-P2A-Venus-rpEF1a-Zeo

Plasmid

#169174

Purpose

piggyBac transposon to generate a Tet inducible SOX17 expressing cell line. Tet-On inducible when paired with #169175

Depositor

Igor Slukvin

Article

Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

Insert

SOX17-P2A-Venus (SOX17 Human, Synthetic)

Use

Piggybac transposon

Expression

Mammalian

Promoter

pTREtight (tet-inducible promoter)

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEF1a-EGFP-miR-MCS (JDW 1478)

Plasmid

#242580

Purpose

An EF1a driven expression vector containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP intron and miR-MCS (empty)

Expression

Mammalian

Promoter

human EF1a

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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