Addgene

We narrowed to 256 results for: h cas9 protein

Showing: 121 - 140 of 256 results

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pNS19-LV-mutRFP-2A-GFP

Plasmid

#113716

Purpose

Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

mutRFP-2A-GFP-IRES-Puro

Use

Lentiviral; Reporter system

Tags

Expression

Mammalian

Mutation

Y64L (mutRFP)

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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MLM3636-Prnp-CDS-Pos

Plasmid

#61857

Purpose

Expresses a gRNA plasmid targeting the prion gene's exon 3, positive strand

Depositor

Gerold Schmitt-Ulms

Article

Mehrabian et al PLoS One. 2014 Dec 9;9(12):e114594. doi:

Insert

PrP guiding sequence (Prnp Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6 promoter

Available since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only
MLM3636-Prnp-CDS-Neg

Plasmid

#61856

Purpose

Expresses a gRNA plasmid targeting the prion gene's exon 3, negative strand

Depositor

Gerold Schmitt-Ulms

Article

Mehrabian et al PLoS One. 2014 Dec 9;9(12):e114594. doi:

Insert

PrP guiding sequence (Prnp Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6 promoter

Available since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only
pCF708

Plasmid

#121685

Purpose

EF1a-dTEV-protease-mTagBFP2

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

TEV protease

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a core promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCF736

Plasmid

#121893

Purpose

EF1a-dTEV-protease-GFP

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

TEV protease
eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF-1a core promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pPV-H1-ccdB-mEF1α-RiH

Plasmid

#100598

Purpose

piggyBac vector for sgRNA cloning

Depositor

Akitsu Hotta

Article

Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

Type

Empty backbone

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Human H1 PolIII promoter, human FTH1 promoter, mo…

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_TIA1

Plasmid

#106097

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA TIA1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_TIAL1

Plasmid

#106096

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA TIAL1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target1 (Mpphot)

Plasmid

#186728

Purpose

Binary vector for CRISPR/Cas9 (target 1: Mpphot [positive control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE010-Target2 (Mpphot)

Plasmid

#186729

Purpose

Binary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).

Depositor

Yutaka Kodama

Article

Konno et al Biochem Biophys Res Commun. 2018 Sep 3;5

Insert

Mphot

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEJS540-pCSDest2-AcrIIC4Hpa

Plasmid

#113434

Purpose

Expresses type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hCRY1 c-term

Plasmid

#179453

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human CRY1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #1

Plasmid

#179454

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #2

Plasmid

#179455

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCrisprV2 sgRNA hPER2c-term #3

Plasmid

#179456

Purpose

Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

sgRNA targeting human PER2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-AlbEx14-mNG

Plasmid

#201780

Purpose

knocks in mNeonGreen into exon 14 of Alb to produce Alb-mNeonGreen fusion protein in mice

Depositor

Hajime Hirase

Article

Vittani et al Fluorescence Imaging of the Brain. Neuro

Inserts

Alb gRNA (Alb Mouse)
Alb_LHA-mNeonGreen-Alb_RHA (Alb Mouse)

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.1

Plasmid

#91895

Purpose

sgNf1.1 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.1

Use

Lentiviral and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.4

Plasmid

#92029

Purpose

sgNf1.4 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.4 for Nf1 deletion

Use

Lentiviral and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCriprV2-sgRNA-PER1-#1

Plasmid

#189987

Purpose

Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Period1 (PER1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCriprV2-sgRNA-PER1-#2

Plasmid

#189988

Purpose

Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Period1 (PER1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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