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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 292 results for: h cas9 protein

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Showing: 121 - 140 of 292 results

  7. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

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  8. pMJ923

    Plasmid
    #78313
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-mCherry-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78313-plasmid-map-sequence-id-148391

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  9. TetO-FUW-VdC9BV

    Plasmid
    #62195
    Purpose
    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes
    Depositor
    Kam Leong
    Article
    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7
    Insert
    VP64dCas9BFPVP64
    Use
    Lentiviral
    Tags
    Two VP64s tagged to dCas9 fused to BFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive) Cas9 (dCas9)
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62195-plasmid-map-sequence-id-101643

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  17. pNS19-LV-mutRFP-2A-GFP

    Plasmid
    #113716
    Purpose
    Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)
    Depositor
    Gerald Schwank
    Article
    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1
    Insert
    mutRFP-2A-GFP-IRES-Puro
    Use
    Lentiviral; Reporter system
    Expression
    Mammalian
    Mutation
    Y64L (mutRFP)
    Available Since
    Aug. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113716-plasmid-map-sequence-id-220030

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Showing: 121 - 140 of 292 results