We narrowed to 2,375 results for: pcas9

1. SpCas9 TadCBEe

   Plasmid

   #193836

   Purpose

   Express TadCBEe (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-CDe-SpCas9D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Mammalian_SpCas9_Target-AID2S

   Plasmid

   #188648

   Purpose

   Expresses SpCas9_Target-AID2S in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID2S-UGI
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SpCas9

   Promoter

   pCMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMA-SpCas9-g5

   Plasmid

   #80788

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV_Efs_hSpCas9_NLS_FLAG-SV40

   Plasmid

   #97307

   Purpose

   AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   humanized S. pyogenes Cas9
   

   Use

   AAV and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMA-SpCas9-g6

   Plasmid

   #80789

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDNR-SpCas9

   Plasmid

   #80425

   Purpose

   Used for in vitro transcription of SpCas9 mRNA.

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   none

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459_gRNA-AAVS1_hspCas9

   Plasmid

   #193309

   Purpose

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6 AAVS1 sgRNA (V2.0)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   not applicable

   Promoter

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mammalian_SpCas9_Target-AID3S

   Plasmid

   #188649

   Purpose

   Expresses SpCas9_Target-AID3S in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID3S-UGI
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SpCas9

   Promoter

   pCMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

   Plasmid

   #97313

   Purpose

   Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available Since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-NG-CGBE1

    Plasmid

    #198537

    Purpose

    Expresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-CGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP

    Plasmid

    #198863

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human TERT gene

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    TERT sgRNA (TERT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    u6

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO5d.EFS.SpCas9.P2A.PAC

    Plasmid

    #58329

    Purpose

    Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    EFS (EEF1A1 Human)
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX165-eSpCas9

    Plasmid

    #140564

    Purpose

    Expresses eSpCas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    K848A K1003A R1060A

    Promoter

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. P628_SpCas9-RecA

    Plasmid

    #87263

    Purpose

    Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    no

    Promoter

    CBh

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. eSpCas9-EF-5Nanog_2

    Plasmid

    #174871

    Purpose

    CRISPR vector for generating Nanog STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Nanog (Nanog Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMA-SpCas9-g7

    Plasmid

    #80790

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SpCas9 TadCBEc

    Plasmid

    #193834

    Purpose

    Express TadCBEc (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDc-SpCas9 D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9-plus

    Plasmid

    #126767

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. eSpCas9-EF-5Pou5f1

    Plasmid

    #174872

    Purpose

    CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-T3-eSpCAS9-NG-pA

    Plasmid

    #131466

    Purpose

    Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

    Insert

    Codon optimized eSpCas9-NG
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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