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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,592 results for: Met;

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Showing: 1381 - 1400 of 19592 results

  3. AlkB-D135G

    Plasmid
    #160434
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Gly
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Glycine; deleted the…
    Promoter
    T7
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160434-plasmid-map-sequence-id-318954

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  15. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

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  17. pEvol-MjaYRS

    Plasmid
    #153557
    Purpose
    Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli
    Depositor
    Huiwang Ai
    Article
    Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038
    Inserts
    MJaYRS (first copy)
    MJaYRS (second copy)
    amber suppression tRNA under proK promoter
    Expression
    Bacterial
    Promoter
    glnS and pBAD
    Available Since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    153557-plasmid-map-sequence-id-306290

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  20. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available Since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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Showing: 1381 - 1400 of 19592 results