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alpha-0 CFP in pcDNA3.1

Plasmid

#36396

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted into the KpnI-SpeI sites engineered …

Promoter

cmv

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
Th-iCre

Plasmid

#159459

Purpose

Targeting vector to TH gene locus for induction of P2A-fused iCre, with PGK-puroR drug resistant cassette flanked by FRT sequence.

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Insert

P2A-iCre

Use

Cre/Lox

Tags

P2A

Expression

Mammalian

Promoter

Endogenous TH

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD4 (OTU, aa 22-196)

Plasmid

#61413

Purpose

Expresses human OTUD4 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-21 and aa 197-1114.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x

Plasmid

#182256

Purpose

Human PDAP1 3` UTR region mutated in 4x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene mutated in 4 binding sites for mir-150

Use

Luciferase

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

Plasmid

#61582

Purpose

Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

Cezanne2 (OTUD7A Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Isoform 2. Deleted aa 463-933.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-Sintbad

Plasmid

#234668

Purpose

expresses SINTBAD (TBKBP1) in mammalian cells

Depositor

Lienhard Schmitz

Article

Saul et al iScience. 2019 Sep 27;19:527-544. doi: 1

Insert

SINTBAD (TBKBP1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pEMS2144

Plasmid

#104357

Purpose

AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Brain. 2016 May 10;9(1):52. doi: 10.

Insert

ssAAV-smCBA-EmGFP

Use

AAV

Promoter

smCBA

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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β₂AR-mCFP

Plasmid

#38260

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

β₂AR (ADRB2 Human)

Tags

Flag, mCFP, and signal peptide sequence (MKTIIALS…

Expression

Mammalian

Mutation

diglycine linker (replacing terminator codon)

Promoter

CMV

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-p53 2KR

Plasmid

#78799

Purpose

To overexpress p53 2KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

p53 (TP53 Human)

Tags

HA

Expression

Mammalian

Mutation

K291R, K292R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pCB19

Plasmid

#194757

Purpose

EGFP-tagged GluRIIA-Construct for integration at attP sites

Depositor

Sven Dannhäuser

Article

Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

Insert

GluRIIA (GluRIIA Fly)

Use

Phic31-integration vector

Tags

EGFP-Tag

Mutation

none

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCB20

Plasmid

#194758

Purpose

ALFA-tagged GluRIIA-Construct for integration at attP sites

Depositor

Sven Dannhäuser

Article

Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

Insert

GluRIIA (GluRIIA Fly)

Use

Phic31-integration vector

Tags

ALFA-Tag

Mutation

none

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS1932

Plasmid

#79781

Purpose

High copy number pUC vector containing Ple259 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple259

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple259 (PAX6 MiniPromoter)

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1937

Plasmid

#79782

Purpose

High copy number pUC vector containing Ple260 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple260

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple260 (PAX6 MiniPromoter)

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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MIGR1-cyclin E-AA

Plasmid

#47498

Purpose

MSCV-based vector encodes human cyclin E mutated within N- and C-terminal Cdc4 phosphodegrons

Depositor

Alex Minella

Article

Xu et al Oncogene. 2013 Jul 22. doi: 10.1038/onc.

Insert

cyclin E1 (CCNE1 Human)

Use

Retroviral; Mscv-ires-gfp

Expression

Mammalian

Mutation

threonines 62 and 380 to alanine (T62A T380A)

Promoter

LTR

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only
pLVX-Phi3-Zsgreen-PGK-mCherry

Plasmid

#153320

Purpose

Expresses zsGreen and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-ANLN_sgRNA

Plasmid

#183874

Purpose

pX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ANLN sgRNA spacer (ANLN Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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1037 pGL3 FasL promoter

Plasmid

#9028

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

Fas ligand promoter (FASLG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
Beta2-AR-TYY

Plasmid

#32694

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2006 Jan 13;281(2):1261-73.

Insert

β2AR (ADRB2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

T68F, Y132G Y219A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pX459-HypaCas9-ECT2_sgRNA

Plasmid

#183873

Purpose

pX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ECT2 sgRNA spacer (ECT2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRELA

Plasmid

#83944

Purpose

Lentiviral vector expressing an sgRNA targeting RELA NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgRELA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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