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pCAGEN-rtTA-IRES-nls-EGFP-WPRE-bGH-pA (JDW 410)

Plasmid

#229807

Purpose

A CAGGS driven tet-on transactivator with an nls-EGFP reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

rtTA-IRES-nls-EGFP

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)

Plasmid

#229812

Purpose

A CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nls-mCherry

Tags

SV40 NLS

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

Plasmid

#229839

Purpose

A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Fli1-zFUCCI-IRES-mTagBFP2 (JDW 1517)

Plasmid

#242602

Purpose

A Tol2 expression vector containing the fli promoter driving FUCCI to label cell cycle in all blood vessels followed by an IRES-mTagBFP2.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGem-P2A-mCherry-zCdt1, nls-mTagBFP2

Use

Zebrafish expression

Promoter

fli promoter

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-IRES-myr-mKate2 (JDW 1503)

Plasmid

#242598

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mKate2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-IRES-myr-mTagBFP (JDW 1502)

Plasmid

#242597

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mTagBFP2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-BLAST-CMV-LiCre-WPRE (JDW 1495)

Plasmid

#242594

Purpose

Lentiviral vector with a CMV promoter driving a light inducible cre (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-mm-miR-500 (JDW 8)

Plasmid

#242563

Purpose

Gateway middle entry clone containing an EGFP fused to the murine microRNA 500.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

GFP with artificial intron and murine microRNA 500 (Mir500 Mouse)

Use

Gateway subcloning

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-Luciferase-WPRE-bGH-pA (JDW 1509)

Plasmid

#242572

Purpose

Gateway 3' entry clone containing an IRES Luciferase

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-Luciferase-WPRE-bGH-pA

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Rox-nlsKate5V-stop-Rox-myr-Flag-BFP-NEO (JDW 473)

Plasmid

#242587

Purpose

A CAGGS driven, Dre recombinase dependent switch reporter (nls-mKate2 to MbBFP following Dre recombination).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nlsKateV5, MbBPF-FLAG, FRTNeoFRT

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a-EGFP-hs-miR-126 (JDW 1415)

Plasmid

#242581

Purpose

An EF1a driven expression vector containing an EGFP fused to the human microRNA 126.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with an intron containing hs-miR-126 (MIR126 Human)

Expression

Mammalian

Promoter

human EF1a

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Actin-Vhh-mNeonGreen-HA-pA (JDW 1308)

Plasmid

#242589

Purpose

A CAGGS driven expression vector containing an actin nanobody fused to mNeonGreen to label actin.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh (actin nanobody)

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)

Plasmid

#229827

Purpose

A CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcripts

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

3xnls-TagBFP2-MCS-FLAG

Expression

Mammalian

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

Plasmid

#229811

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA transactivator-LexA-Operon-nls-GFP

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

Plasmid

#229823

Purpose

A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

Plasmid

#229824

Purpose

A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

Plasmid

#229837

Purpose

A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

Plasmid

#229838

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp

Use

Tol2 based expression vector

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

Plasmid

#229840

Purpose

A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pUS212

Plasmid

#84580

Purpose

E.coli cloning and expression vector. Has cloning sites downstream of Tet promoter.

Depositor

Nicholas Coleman

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 21, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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