We narrowed to 88,917 results for: cloning
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Plasmid#78571PurposeExpresses untagged human rhinovirus 3C protease in E. coliDepositorAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pSRK2706
Plasmid#78571PurposeExpresses untagged human rhinovirus 3C protease in E. coliDepositorAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Proinsulin-NanoLuc in pLX304
Plasmid#62057PurposeLuminescent reporter of insulin secretionDepositorInsertProinsulin-NanoLuc (Ins2 Mouse, Synthetic)
UseLentiviral and LuciferaseExpressionMammalianMutationInserted NanoLuc into C-peptide of mouse Ins2 cod…PromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Proinsulin-NanoLuc in pLX304
Plasmid#62057PurposeLuminescent reporter of insulin secretionDepositorInsertProinsulin-NanoLuc (Ins2 Mouse, Synthetic)
UseLentiviral and LuciferaseExpressionMammalianMutationInserted NanoLuc into C-peptide of mouse Ins2 cod…PromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64
Plasmid#180263PurposeLentiviral expression vector for dCas9-VP64 in mammalian cellsDepositorInsertdCas9
UseCRISPR and LentiviralExpressionMammalianMutationNuclease dead Cas9PromoterSFFVAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR/D_creERt2
Plasmid#27321PurposeGateway entry vector containing Cre(ERTt), for 4-hydroxytamoxifen-controlled Cre recombinase activityDepositorInsertCreERt2
UseMultisite gateway middle entry vectorAvailable sinceJan. 14, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR/D_creERt2
Plasmid#27321PurposeGateway entry vector containing Cre(ERTt), for 4-hydroxytamoxifen-controlled Cre recombinase activityDepositorInsertCreERt2
UseMultisite gateway middle entry vectorAvailable sinceJan. 14, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX-TRE-dCas9-KRAB-MeCP2-BSD
Plasmid#140690PurposeInducible knockdown of gene expression in human cells for pooled scRNA-seq experimentsDepositorPublicationInsertCas9m4-KRAB-MeCP2
UseCRISPR and LentiviralExpressionMammalianAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiGuide-Hygro-eGFP
Plasmid#99375PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-eGFP
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiGuide-Hygro-eGFP
Plasmid#99375PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-eGFP
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLIK-IDH1-R132H-FLAG
Plasmid#66803PurposeTet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vectorDepositorInsertIDH1 (IDH1 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationR132HPromoterCMVAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLIK-IDH1-R132H-FLAG
Plasmid#66803PurposeTet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vectorDepositorInsertIDH1 (IDH1 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationR132HPromoterCMVAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKI1.1R
Plasmid#85808PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionPlantPromoterAtRPS5AAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKI1.1R
Plasmid#85808PurposeCRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.DepositorInserthuman-codon-optimized SpCas9
UseCRISPRTagsFLAG and NLSExpressionPlantPromoterAtRPS5AAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TFORF3549
Plasmid#145025PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertGFP
UseLentiviralExpressionMammalianPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-MMLVgag-3xNES-Cas9
Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMMLV-Gag-3xNES-Cas9 (Spy)
TagsFlagExpressionMammalianPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET PPL His6 MBP LIC cloning vector (2K-T)
Plasmid#37183DepositorTypeEmpty backboneTagsHis6, MBP, and PPLExpressionBacterialPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-hMLH1dn for IVT
Plasmid#178114PurposeTemplate for in vitro transcription of human MLH1dnDepositorInserthuman MLH1dn (codon optimized) (MLH1 Human)
UseTemplate for in vitro transcriptionMutationDetailed in manuscriptPromoterT7 (inactivated)Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_ACh3.0
Plasmid#121922Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0DepositorHas serviceAAV9InsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_ACh3.0
Plasmid#121922Purposeexpress the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0DepositorHas serviceAAV9InsertGPCR activation based ACh sensor GRAB-ACh3.0
UseAAVPromoterhSynAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by