We narrowed to 59,403 results for: cloning

1. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

   Plasmid

   #229839

   Purpose

   A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-EGFP-mm-miR-500 (JDW 8)

   Plasmid

   #242563

   Purpose

   Gateway middle entry clone containing an EGFP fused to the murine microRNA 500.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   GFP with artificial intron and murine microRNA 500 (Mir500 Mouse)
   

   Use

   Gateway subcloning

   Available Since

   Oct. 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-Rox-nlsKate5V-stop-Rox-myr-Flag-BFP-NEO (JDW 473)

   Plasmid

   #242587

   Purpose

   A CAGGS driven, Dre recombinase dependent switch reporter (nls-mKate2 to MbBFP following Dre recombination).

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   nlsKateV5, MbBPF-FLAG, FRTNeoFRT
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF1a-EGFP-hs-miR-126 (JDW 1415)

   Plasmid

   #242581

   Purpose

   An EF1a driven expression vector containing an EGFP fused to the human microRNA 126.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EGFP with an intron containing hs-miR-126 (MIR126 Human)
   

   Expression

   Mammalian

   Promoter

   human EF1a

   Available Since

   Sept. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

   Plasmid

   #229810

   Purpose

   A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-AmCyan-stop-lox
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

   Plasmid

   #229813

   Purpose

   A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-mODC-LexAOp-cFos
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

   Plasmid

   #229814

   Purpose

   A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-EGFP-stop-lox
   

   Use

   Cre/Lox; Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

   Plasmid

   #229816

   Purpose

   A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   loxp-EGFP-stop-lox
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

   Plasmid

   #229824

   Purpose

   A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase-P2A-H2A-mCherry
   

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

    Plasmid

    #229841

    Purpose

    A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    loxp-H2B-mCerulean-2xStop-loxP
    

    Use

    Cre/Lox; Tol2 based expression vector

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

    Plasmid

    #229838

    Purpose

    A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-mODC-LexAOp
    

    Use

    Tol2 based expression vector

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUS212

    Plasmid

    #84580

    Purpose

    E.coli cloning and expression vector. Has cloning sites downstream of Tet promoter.

    Depositor

    Nicholas Coleman

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 21, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pHyVec13

    Plasmid

    #34796

    Depositor

    Rob Steele

    Type

    Empty backbone

    Use

    Hydra expression

    Tags

    GFP

    Promoter

    Hydra vulgaris actin promoter

    Available Since

    April 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHyVec4

    Plasmid

    #34791

    Depositor

    Rob Steele

    Type

    Empty backbone

    Use

    Hydra expression

    Tags

    GFP

    Promoter

    Hydra vulgaris actin promoter

    Available Since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIA028

    Plasmid

    #200419

    Purpose

    thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning

    Depositor

    Rut Carballido-López

    Article

    Altinoglu et al bioRxiv 2023

    Type

    Empty backbone

    Tags

    PAmCherry1

    Expression

    Bacterial

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG426GPD-ccdB

    Plasmid

    #14156

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Yeast

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG423GAL-ccdB

    Plasmid

    #14149

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Yeast

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG416GPD-ccdB

    Plasmid

    #14148

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Yeast

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG425GPD-ccdB

    Plasmid

    #14154

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Yeast

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG423GPD-ccdB

    Plasmid

    #14150

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Yeast

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge