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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 1381 - 1400 of 11938 results

  3. pcDNA-LBR-mRFP1-polyA

    Plasmid
    #59749
    Purpose
    Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.
    Depositor
    Yi Zhang
    Publication
    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LBR-mRFP1
    Tags
    mRFP1
    Expression
    Mammalian
    Mutation
    aa 1-249 of LBR
    Promoter
    CMV and T7
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59749-plasmid-map-sequence-id-94053

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  9. pAAV_hSyn-PdCO-mScarlet-WPRE (AAV5)

    Viral prep
    #198510-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_hSyn-PdCO-mScarlet-WPRE (#198510). In addition to the viral particles, you will also receive purified pAAV_hSyn-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Publication
    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s
    Promoter
    Syn
    Tags
    mScarlet
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only

  10. VAMP2-12aa-mCit

    Plasmid
    #53237
    Purpose
    Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.
    Depositor
    Robert Zucker
    Publication
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAMP2 (Vamp2 Rat)
    Tags
    12aa-linker and mCitrine
    Expression
    Mammalian
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pETDuet-1 TIM9,10

    Plasmid
    #170280
    Purpose
    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Publication
    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.
    Insert
    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    Expression
    Bacterial
    Promoter
    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170280-plasmid-map-sequence-id-334954

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  16. C1-mCitrine-FKBP-EZRabd(t567D)

    Plasmid
    #155227
    Purpose
    Acute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with Rapamycin
    Depositor
    Tobias Meyer
    Publication
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    Changed Thr 567 to Asp in original Ezrin
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    155227-plasmid-map-sequence-id-310009

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Showing: 1381 - 1400 of 11938 results