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LentiGuide-zeo

Plasmid

#160091

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance marker

Depositor

Rizwan Haq

Publication

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHis-hTG2

Plasmid

#100719

Purpose

Expression of human transglutaminase 2 in E.coli

Depositor

Byung Il Lee

Publication

Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

Insert

TGM2 (TGM2 Human)

Tags

His

Expression

Bacterial

Mutation

silent mutation Arg580 (AGG--CGT) to enhance expr…

Promoter

T7

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLKO5.sgRNA.EFS.PAC

Plasmid

#57825

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
PAC

Use

CRISPR and Lentiviral

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pFA6a-6xGLY-FLAG-kanMX6

Plasmid

#20751

Depositor

Mark Hochstrasser

Publication

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKS diaCas9_sgRNA

Plasmid

#74923

Purpose

Expresses Cas9 codon optimized for Phaeodactylum tricornutum and a sgRNA driven by a U6 promoter

Depositor

Per Winge

Publication

Nymark et al Sci Rep. 2016 Apr 25;6:24951. doi: 10.10

Inserts

LHCF2 promoter
diaCas9
LHCF1 terminator
U6 promoter
sgRNA
U6 3' region

Use

CRISPR

Promoter

Cas9 module and sgRNA module

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pFA6a-6xGLY-His-tag-kanMX6

Plasmid

#20763

Depositor

Mark Hochstrasser

Publication

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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PKC gamma WT

Plasmid

#21236

Depositor

Bernard Weinstein

Publication

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKC gamma (Prkcg Mouse)

Tags

HA

Expression

Mammalian

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
FLAG.PKCepsilon.K/W

Plasmid

#10796

Depositor

Alex Toker

Publication

Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKC epsilon (PRKCE Human)

Tags

FLAG

Expression

Mammalian

Mutation

Kinase dead mutant: K437W (Lys 437 to Trp)

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pattB-QF-hsp70

Plasmid

#24368

Depositor

Liqun Luo

Publication

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QF

Expression

Insect

Available since

May 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCI SMN2

Plasmid

#72287

Purpose

SMN2 exon 7 splicing cassette

Depositor

Elliot Androphy

Publication

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN2 (SMN2 Human)

Expression

Mammalian

Mutation

contains exons 6-7-8 including introns, multiple …

Promoter

CMV

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pSR prdm3 (PRDM16 shRNA)

Plasmid

#15505

Depositor

Bruce Spiegelman

Publication

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 shRNA (Prdm16 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
Su9-EGFP

Plasmid

#23214

Depositor

David Chan

Publication

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Subunit 9 of the F0-ATPase

Tags

EGFP

Expression

Mammalian

Mutation

contains AA 1-69 of subunit 9

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pWZL Neo Myr Flag PRKCD

Plasmid

#20603

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKCD (PRKCD Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
LNCX TbetaRII DN

Plasmid

#12640

Depositor

Rik Derynck

Publication

Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

Insert

TGF-beta receptor II (TGFBR2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: truncated cytoplasmically

Available since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Flag-HA-ATXN3

Plasmid

#22538

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

ATXN3 (ATXN3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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PKC beta I WT

Plasmid

#16378

Depositor

Bernard Weinstein

Publication

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PKC beta 1 (Prkcb Rat)

Tags

HA

Expression

Mammalian

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pBABE HA Chk2 KD, dominant negative chk2

Plasmid

#41902

Depositor

Stephen Elledge

Publication

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

Chk2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D347A

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe.puro.CDK8.KD.flag

Plasmid

#19759

Depositor

William Hahn

Publication

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

D173A (kinase dead)

Available since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) Laccase2 MCS Exon Vector

Plasmid

#69893

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

Depositor

Jeremy Wilusz

Publication

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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GFP-ASH1L

Plasmid

#65369

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ASH1L (ASH1L Human)

Tags

EmGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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