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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,014 results for: αGFP

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Showing: 1421 - 1440 of 2014 results

  1. pSbi-pur-EGFP-KSR1-CA3 (N-KSR)

    Plasmid
    #217769
    Purpose
    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Publication
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Sleeping beauty transposase-compatible genome ins…
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CA3 domain aa 317-400
    Promoter
    EF-1alpha promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217769-plasmid-map-sequence-id-435786

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  8. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV9)

    Viral prep
    #112677-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Publication
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  11. pGA1-EF1α-sfGFP150TAG-4xtRNA

    Plasmid
    #251517
    Purpose
    Expression of sfGFP150TAG using the Methanomethylophilus alvus (Ma) PylRS/tRNA system for site-specific incorporation of noncanonical amino acids at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Publication
    Augustin et al J Am Chem Soc. 2026 Feb 12. doi: 10.1021
    Insert
    sfGFP150TAG
    Tags
    His6 and V5
    Expression
    Mammalian
    Mutation
    150 TAG (amino acid 150 mutated to stop codon)
    Promoter
    EF1alpha
    Available since
    March 12, 2026
    Availability
    Academic Institutions and Nonprofits only
    251517-plasmid-map-sequence-id-502878

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  12. pcDNA3.1(-)-EGFP-AP4-mito

    Plasmid
    #229693
    Purpose
    Transient expression of EGFP AP4-mito in mammalian cells
    Depositor
    Alpha Yap
    Publication
    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP; Control construct with tandem AP4 motifs (mitochondria)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229693-plasmid-map-sequence-id-458303

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  15. FKBP-LBR(1-245)-GFP

    Plasmid
    #222425
    Purpose
    To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells
    Depositor
    Stephen Royle
    Publication
    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LBR(1-245aa) (LBR Human)
    Tags
    FKBP and GFP
    Expression
    Mammalian
    Mutation
    Truncation (Includes aa 1-245)
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    222425-plasmid-map-sequence-id-439254

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  16. pGG-TurboID-GFP-PTS1

    Plasmid
    #209406
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta
    Depositor
    Rosa Lozano-Durán
    Publication
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    3XHA-TurboID-PTS1
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209406-plasmid-map-sequence-id-418444

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  17. GFP Cav2.2 HA D122A pcDNA3

    Plasmid
    #206120
    Purpose
    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1
    Depositor
    Annette Dolphin
    Publication
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cacna1b (CACNA1B Rabbit)
    Expression
    Mammalian
    Mutation
    D122A
    Promoter
    CMV
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    206120-plasmid-map-sequence-id-415673

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  18. GFP Cav2.2 HA D665A pcDNA3

    Plasmid
    #206113
    Purpose
    expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D665A mutation that allows trafficking to the plasma membrane but channel is not functional
    Depositor
    Annette Dolphin
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cacna1b (CACNA1B Rabbit)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    D665A
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206113-plasmid-map-sequence-id-415392

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  19. GFP Cav2.2 HA L702P pcDNA3

    Plasmid
    #206056
    Purpose
    expression of rabbit Cav2.2 with a N-terminal GFP tag and an exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functional
    Depositor
    Annette Dolphin
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cacna1b (CACNA1B Rabbit)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    L702P
    Promoter
    CMV
    Available since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    206056-plasmid-map-sequence-id-409942

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Showing: 1421 - 1440 of 2014 results