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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,352 results for: Cas9

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Showing: 1421 - 1440 of 14352 results

  2. pSL1394

    Plasmid
    #129522
    Purpose
    pDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd Generation
    Depositor
    Scott Lindner
    Publication
    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Use
    Plasmodium, rodent-infectious species
    Tags
    3XFLAG-NLS
    Available since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129522-plasmid-map-sequence-id-256153

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  4. pPB9v2

    Plasmid
    #210029
    Purpose
    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    Cas9
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210029-plasmid-map-sequence-id-414001

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  13. pYC2085

    Plasmid
    #158721
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Publication
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158721-plasmid-map-sequence-id-320843

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  14. pCSN061

    Plasmid
    #101725
    Purpose
    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
    Depositor
    Rene Verwaal
    Publication
    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278
    Inserts
    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Promoter
    Heterologous TEF1 promoter from A. gossypii. and …
    Available since
    Dec. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101725-plasmid-map-sequence-id-199888

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  17. pEPQD1CB0202

    Plasmid
    #185626
    Purpose
    MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter
    Depositor
    Nicola Patron
    Publication
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9
    Use
    CRISPR and Synthetic Biology
    Available since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185626-plasmid-map-sequence-id-368553

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  20. pX458M-53BP1-DN1S

    Plasmid
    #131045
    Purpose
    Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.
    Depositor
    Punam Malik
    Publication
    Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-53BP1-DN1S (TP53BP1 Human)
    Use
    CRISPR
    Tags
    GFP and SpCas9
    Expression
    Mammalian
    Mutation
    Only expressing residues 1231-1644 of complete 53…
    Promoter
    CBH
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    131045-plasmid-map-sequence-id-258265

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Showing: 1421 - 1440 of 14352 results