We narrowed to 2,171 results for: Pam

1. pMXS-IRES-BLAST flag-CAD R1398A A1400E R1403A R1404A L1405A

   Plasmid

   #188138

   Purpose

   Expresses C-terminal flag-tagged human CAD R1398A A1400E R1403A R1404A L1405A in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Phage-PGK-Flag-HA-FKBP-T(G177D)

   Plasmid

   #181735

   Purpose

   for lentiviral expression of brachyury ORF in mammalian cells

   Depositor

   Charles Lin

   Article

   Sheppard et al Cell Rep Med. 2021 Jan 19;2(1):100188. d

   Insert

   T (brachyury)
   

   Use

   Lentiviral

   Mutation

   a silent mutation in the PAM site of an sgRNAt ta…

   Available Since

   March 21, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

   Plasmid

   #139999

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

   Plasmid

   #140002

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

   Plasmid

   #139989

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV.tetO.Ascl1.U6.shRest1.U6.shRest2

   Plasmid

   #234846

   Purpose

   3rd generation lentiviral vector, expresses Ascl1 under control of TetON promoter and two shREST sequences under control of U6 promoters

   Depositor

   Malin Parmar

   Article

   Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro

   Plasmid

   #176484

   Purpose

   TetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   Lmx1A-T2A-Pitx3 (PITX3, LMX1A Human)
   

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

   Plasmid

   #169128

   Purpose

   AAV plasmid containing a mKate2 and a truncated PDE4D3

   Depositor

   Georg Nagel

   Article

   Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

   Has Service

   AAV1

   Insert

   mKate2-PDE4DCatL (PDE4D Human)
   

   Use

   AAV and Cre/Lox

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   truncated, Catalatic domain only

   Promoter

   EF1a

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

   Plasmid

   #140004

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FRB_T2098L-VAMP3-mCherry

    Plasmid

    #92429

    Purpose

    Expression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    VAMP3 (Vamp3 Mouse)
    

    Tags

    FKBP rapamycin binding (FRB) domain of mTOR with …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFB-EF1a-DIO-Rpl22l1-3xFLAG-2A-GFP

    Plasmid

    #245377

    Purpose

    Cre-dependent expression of FLAG-tagged Rpl22l1 and EGFP.

    Depositor

    D. James Surmeier

    Article

    Geibl et al Mol Neurodegener. 2024 Oct 8;19(1):69. d

    Insert

    Rpl22l1 (Rpl22l1 Mouse)
    

    Use

    AAV

    Tags

    3xFLAG, T2A-GFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. phH1-GBX2-gRNA

    Plasmid

    #192510

    Purpose

    Contains gRNA for GBX2

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    GBX2 (GBX2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. phU6-CDX4-gRNA

    Plasmid

    #192511

    Purpose

    Contains gRNA for CDX4

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    CDX4 (CDX4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmU6-CDX1-gRNA

    Plasmid

    #192512

    Purpose

    Contains gRNA for CDX1

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    CDX1 (CDX1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. ph7SK-CDX2-gRNA

    Plasmid

    #192513

    Purpose

    Contains gRNA for CDX2

    Depositor

    Mark Denham

    Article

    Maimaitili et al Nat Commun. 2023 Dec 5;14(1):7871. doi:

    Insert

    CDX2 (CDX2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid

    #140003

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

    Plasmid

    #160141

    Purpose

    Cre- dependent expression of Sun1GFP for nuclei isolation

    Depositor

    Andreas Pfenning

    Article

    Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

    Insert

    Sun1-GFP (Sun1 Mouse)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N-truncated: removed amino acids 1-207, retained …

    Promoter

    Ef1a

    Available Since

    March 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid

    #139997

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PiggyBac-TetO-En1-t2a-Foxa2-hygro

    Plasmid

    #176483

    Purpose

    TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    En1-t2a-FoxA2 (FOXA2, En1 Human, Mouse)
    

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid

    #139996

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge