We narrowed to 5,839 results for: pCas

1. pCas9-EcRT-Z3-Kan

   Plasmid

   #232096

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   Z3

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA

   Plasmid

   #225350

   Purpose

   piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression

   Depositor

   Wendell Lim

   Article

   Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

   Insert

   H2B (H2B Human, Synthetic)
   

   Tags

   miRFP and non-fluorescent GFP (Y67F)-P2A

   Expression

   Mammalian

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-CAPN7-OSF

   Plasmid

   #193041

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Promoter

   Chicken Beta-actin

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA528 CAPN7 (81-165)

   Plasmid

   #193032

   Purpose

   Bacterial expression plasmid for CAPN7 (81-165) C-terminal MIT domain

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   Residues 81-165

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-nucGFP11-3xPax7gRNA

   Plasmid

   #224570

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCambia3301-AtU6-MAR1

   Plasmid

   #210757

   Purpose

   Mutagenesis of the Arabidopsis MAR1 gene for efficient screening.

   Depositor

   Daisuke Miki

   Article

   Cheng et al Sci Rep. 2024 Dec 28;14(1):30791. doi: 1

   Insert

   AtU6-26 pro::MAR1 sgRNA
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU6-26

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-pCAG-mock

   Plasmid

   #228504

   Purpose

   This plasmid can be used for control experiments and can also be used as a plasmid backbone

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-protein-mock

   Plasmid

   #228505

   Purpose

   This plasmid can be used for control experiments and can also be used as a plasmid backbone

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCASCADE-LCAmp-trxB

   Plasmid

   #202465

   Purpose

   Expresses a guide RNA to silence trxB after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   trxB guide RNA
   

   Expression

   Bacterial

   Promoter

   ugpB

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-BiPOLES-mRuby3

    Plasmid

    #227780

    Purpose

    Optogenetic tool for blue-light inhibition and red-light excitation of neurons fused to mRuby3

    Depositor

    Hillel Adesnik

    Insert

    BiPOLES-mRuby3
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-NRCH-CGBE1

    Plasmid

    #198557

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-CGBE1
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpCas9-NG-ABE8.17-m+V106W

    Plasmid

    #198534

    Purpose

    Expresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-ABE8.17-m+V106W
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-AGG-SpCas9

    Plasmid

    #214603

    Purpose

    encode SpCas9; in vitro transcribed modified RNA vector

    Depositor

    Yuxuan Guo

    Article

    Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

    Insert

    SpCas9
    

    Use

    CRISPR and Unspecified

    Promoter

    T7

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2T_CAG_SpCas9PE2_Igf1-NFATC2IP_BlastR (pepPE2_3)

    Plasmid

    #173067

    Purpose

    Expresses NI-PE2

    Depositor

    Richard Sherwood

    Article

    Velimirovic et al Nat Commun. 2022 Jun 18;13(1):3512. doi:

    Insert

    Igf1-NFATC2IP
    

    Expression

    Mammalian

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG_SRGAP2A_SH3(W765A)_GFP

    Plasmid

    #222616

    Purpose

    Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

    Depositor

    Franck Polleux

    Article

    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

    Insert

    SRGAP2 (SRGAP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation W765A (SH3 domain)

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG_SRGAP2A_SH3(W765A)_HA

    Plasmid

    #222614

    Purpose

    Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

    Depositor

    Franck Polleux

    Article

    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

    Insert

    SRGAP2 (SRGAP2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Point mutation W765A (SH3 domain)

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX459v3-eSpCas9(1.1)

    Plasmid

    #178800

    Purpose

    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX459v3-SpCas9-HF1

    Plasmid

    #178801

    Purpose

    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSAVED-CHAT-cbm_PCaspase

    Plasmid

    #214047

    Purpose

    Bacterial expression plasmid for SAVED-CHAT cA3 binding pocket mutant and PCaspase from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Expression

    Bacterial

    Mutation

    K121E, Q122A, N274A, R276E, Y285A, F286A

    Promoter

    lacUV5 promoter

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSAVED-CHAT_PCaspase-N-term

    Plasmid

    #214044

    Purpose

    Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Expression

    Bacterial

    Mutation

    aa 1-153

    Promoter

    lacUV5 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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