We narrowed to 8,654 results for: pcDNA3

1. pcDNA3.1 FLII12Pglu-700uDelta6

   Plasmid

   #17866

   Depositor

   Wolf Frommer

   Article

   Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

   Insert

   FLII12Pglu-700uDelta6
   

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   700uDelta6, ECFP insert in mglB

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Dio-GFP

   Plasmid

   #198279

   Purpose

   Cre reporter for mammalian cells; double-floxxed-EGFP

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Dio-GFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-endo-Nb37

   Plasmid

   #205514

   Purpose

   Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-Nb37
   

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-GGamma13-GFP2

   Plasmid

   #140992

   Purpose

   Encodes a G gamma subunit (GNG13) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma13-GFP2 (GNG13 Human)
   

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-PM-ExRai-AktAR2

   Plasmid

   #184050

   Purpose

   Plasma membrane-targeted ExRai-AktAR2

   Depositor

   Jin Zhang

   Article

   Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

   Insert

   PM-ExRai-AktAR2
   

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-SARS-Spike

   Plasmid

   #145031

   Purpose

   Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   SARS-CoV Spike
   

   Tags

   C9 and CD5 signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 16, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA3.1(-)-Green Falcan0.3

   Plasmid

   #199704

   Purpose

   green fluorescent indicator for cAMP imaging

   Depositor

   Tetsuya Kitaguchi

   Article

   Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

   Insert

   Green Falcan0.3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(-)-Green Falcan3

   Plasmid

   #199706

   Purpose

   green fluorescent indicator for cAMP imaging

   Depositor

   Tetsuya Kitaguchi

   Article

   Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

   Insert

   Green Falcan3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcdna3 flag - ULK1 wt

   Plasmid

   #27636

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse ULK1 (Ulk1 Mouse)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Initial M from ULK1 removed.

   Available Since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Gαi*-BERKY3

    Plasmid

    #158198

    Purpose

    BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3-endo-Nb80

    Plasmid

    #205518

    Purpose

    Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-Nb80
    

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(-)-Green Falcan1

    Plasmid

    #199705

    Purpose

    green fluorescent indicator for cAMP imaging

    Depositor

    Tetsuya Kitaguchi

    Article

    Hiasa et al RSC Adv. 2023 May 22;13(23):15514-15520.

    Insert

    Green Falcan1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-TLR1-YFP

    Plasmid

    #13014

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 1 (TLR1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-FRB-LgBiT

    Plasmid

    #182650

    Purpose

    FRB-LgBiT

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    FRB-LgBiT fusion
    

    Tags

    LgBiT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Xiap-Myc

    Plasmid

    #11833

    Depositor

    Guy Salvesen

    Article

    Takahashi et al J Biol Chem. 1998 Apr 3. 273(14):7787-90

    Insert

    XIAP (XIAP Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-hSTING (S366A)

    Plasmid

    #214155

    Purpose

    Transient expression of STING

    Depositor

    Nan Yan

    Article

    Wu et al Immunity. 2020 Jul 14;53(1):115-126.e5.

    Insert

    STING (S366A) (STING1 Human)
    

    Expression

    Mammalian

    Mutation

    S366A

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. ELP-TEMP/pcDNA3.1(-)

    Plasmid

    #178434

    Purpose

    Genetically encoded ratiometric fluorescent thermometer

    Depositor

    Takeharu Nagai

    Article

    Vu et al Sci Rep. 2021 Aug 13;11(1):16519. doi: 1

    Insert

    ELP-TEMP
    

    Tags

    No tag

    Expression

    Mammalian

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-endo-GsCT

    Plasmid

    #205516

    Purpose

    Endosome-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-GsCT (GNAS Human, Synthetic)
    

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-mitoExRai-AKAR2

    Plasmid

    #161757

    Purpose

    ExRai-AKAR2 targeted to the outer mitochondrial membrane.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    DAKAP-ExRai-AKAR2
    

    Tags

    N-terminal 30 amino acids from DAKAP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1+FLAG-KrascomG12D

    Plasmid

    #206844

    Purpose

    To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codons altered to most optimum based on mouse cod…

    Available Since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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