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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 26,133 results for: Nes;

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Showing: 2881 - 2920 of 26133 results

  1. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid
    #103866
    Purpose
    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103866-plasmid-map-sequence-id-199147

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  2. pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)

    Plasmid
    #103864
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103864-plasmid-map-sequence-id-199176

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  3. pAB1676 REPAIR.t1

    Plasmid
    #176319
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176319-plasmid-map-sequence-id-345279

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  7. pαH-S-RRAR/XBB.1.5

    Plasmid
    #212993
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein of Omicron.XBB.1.5 with with furin side intact
    Depositor
    Priyamvada Acharya
    Article
    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.
    Insert
    Spike S-RRAR/XBB.1.5 (S )
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); furin site intake; (T19I…
    Promoter
    CMV
    Available Since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    212993-plasmid-map-sequence-id-423196

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  9. pUAP1

    Plasmid
    #63674
    Purpose
    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
    Depositor
    Nicola Patron
    Article
    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np
    Insert
    RFP cloning selection cassette
    Use
    Synthetic Biology
    Available Since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63674-plasmid-map-sequence-id-106088

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  10. pKT-IDH1(R132H)-IRES-Katushka

    Plasmid
    #124257
    Purpose
    Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Article
    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii
    Insert
    Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
    Expression
    Mammalian
    Mutation
    Arginine 132 is mutated to Histidine. This mutati…
    Available Since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124257-plasmid-map-sequence-id-252091

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  11. 4xNFkB Luc

    Plasmid
    #111216
    Purpose
    reporter gene
    Depositor
    Johannes A. Schmid
    Insert
    firefly Luciferase
    Expression
    Mammalian
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111216-plasmid-map-sequence-id-213082

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  12. pVenus-N1 (804)

    Plasmid
    #61854
    Purpose
    empty backbone for tagging proteins at the C-terminus with Venus
    Depositor
    Jonathon Pines
    Type
    Empty backbone
    Tags
    Venus
    Expression
    Mammalian
    Available Since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61854-plasmid-map-sequence-id-375257

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  13. pcDNA3.1-xASIC1

    Plasmid
    #32793
    Depositor
    Cecelia Canessa
    Insert
    ASIC1
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 1, 2011
    Availability
    Academic Institutions and Nonprofits only

  14. FUCas9GFP

    Plasmid
    #85555
    Purpose
    Expresses Cas9 with green fluorescent reporter
    Depositor
    Marco Herold
    Insert
    humanised S.pyogenes Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    hUBC
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85555-plasmid-map-sequence-id-169910

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  15. FUCas9Cyan

    Plasmid
    #85554
    Purpose
    Expresses Cas9 with fluorescent Cyan reporter
    Depositor
    Marco Herold
    Insert
    humanised S.pyrogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    hUBC
    Available Since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85554-plasmid-map-sequence-id-180892

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  16. pEN_TT luciferase-V5

    Plasmid
    #220360
    Purpose
    Gateway entry vector for an inducible V5-tagged luciferase
    Depositor
    Kevin Janes
    Insert
    luciferase
    Use
    Entry vector for gateway cloning
    Tags
    V5
    Available Since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220360-plasmid-map-sequence-id-447352

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  17. EGFP-TRAF1

    Plasmid
    #111199
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TRAF1 (TRAF1 Human)
    Tags
    EGFP-FLAG
    Expression
    Mammalian
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111199-plasmid-map-sequence-id-213113

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  18. EYFP-TRAF1

    Plasmid
    #111200
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TRAF1 (TRAF1 Human)
    Tags
    EYFP and FLAG
    Expression
    Mammalian
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111200-plasmid-map-sequence-id-212973

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  19. ECFP-TRAF1

    Plasmid
    #111198
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TRAF1 (TRAF1 Human)
    Tags
    ECFP and FLAG
    Expression
    Mammalian
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111198-plasmid-map-sequence-id-213171

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  21. pCMK

    Plasmid
    #62818
    Purpose
    Bacterial constitutive S. pyogenes Cas9 expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    Cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    62818-plasmid-map-sequence-id-114423

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  22. pcDNA3.1-aASIC1

    Plasmid
    #32795
    Depositor
    Cecelia Canessa
    Insert
    ASIC1
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. pTet_KaeCanABC

    Plasmid
    #248953
    Purpose
    CanABC from K. aerogenes under control of pTet and the native promoter
    Depositor
    Jennifer Doudna
    Insert
    KaeCanABC
    Expression
    Bacterial
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248953-plasmid-map-sequence-id-500768

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  24. pX330-Flag-eSpCas9-D10A

    Plasmid
    #80449
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_D10A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80449-plasmid-map-sequence-id-157945

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  25. pX330-Flag-eSpCas9-H840A

    Plasmid
    #80454
    Purpose
    enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    ehSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    80454-plasmid-map-sequence-id-151472

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  26. pSLIK luciferase-V5 neo

    Plasmid
    #220364
    Purpose
    Lentiviral expression vector for an inducible V5-tagged luciferase construct
    Depositor
    Kevin Janes
    Insert
    luciferase
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220364-plasmid-map-sequence-id-436883

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  27. pX330-Flag-HFSpCas9-H840A

    Plasmid
    #80455
    Purpose
    high fidelity SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80455-plasmid-map-sequence-id-151476

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  28. EGFP-AR

    Plasmid
    #111215
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    AR (Ar Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111215-plasmid-map-sequence-id-264404

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  29. TNFR2-GFP

    Plasmid
    #111207
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TNFRSF1B (TNFRSF1B Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S175P, D306G
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111207-plasmid-map-sequence-id-213090

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  31. TNFR2-YFP

    Plasmid
    #111210
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Insert
    TNFRSF1B (TNFRSF1B Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    S175P, D306G
    Available Since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    111210-plasmid-map-sequence-id-212997

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  32. pCR2.1-hASIC1

    Plasmid
    #32790
    Depositor
    Cecelia Canessa
    Insert
    ASIC1 (ASIC1 Human)
    Use
    Cloning
    Promoter
    plac
    Available Since
    Nov. 1, 2011
    Availability
    Academic Institutions and Nonprofits only

  33. Pv-FDPS

    Plasmid
    #25554
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pv-FDPS
    Tags
    His
    Expression
    Bacterial
    Available Since
    Sept. 10, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. pX330-Flag-HeFSpCas9-H840A

    Plasmid
    #80456
    Purpose
    high fidelity enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HeFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Aug. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80456-plasmid-map-sequence-id-151478

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  35. pTet_KaeCanABC(G189K)

    Plasmid
    #248957
    Purpose
    CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (G189K)
    Depositor
    Jennifer Doudna
    Insert
    KaeCanABC(G189K)
    Expression
    Bacterial
    Mutation
    G189K
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248957-plasmid-map-sequence-id-500783

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  36. pTet_KaeCanA(C187A)BC

    Plasmid
    #248954
    Purpose
    CanABC from K. aerogenes under control of pTet and the native promoter, with CanA mutated (C187A)
    Depositor
    Jennifer Doudna
    Insert
    KaeCanA(C187A)BC
    Expression
    Bacterial
    Mutation
    C187A
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248954-plasmid-map-sequence-id-500775

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  37. pTet_KaeCanABC(G104K)

    Plasmid
    #248956
    Purpose
    CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (G104K)
    Depositor
    Jennifer Doudna
    Insert
    KaeCanABC(G104K)
    Expression
    Bacterial
    Mutation
    G104K
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248956-plasmid-map-sequence-id-500766

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  38. pTet_KaeCanABC(H271A)

    Plasmid
    #248955
    Purpose
    CanABC from K. aerogenes under control of pTet and the native promoter, with CanC mutated (H271A)
    Depositor
    Jennifer Doudna
    Insert
    KaeCanABC(H271A)
    Expression
    Bacterial
    Mutation
    H271A
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248955-plasmid-map-sequence-id-500762

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  39. exp_KaeCanAB

    Plasmid
    #248959
    Purpose
    His-MBP-CanAB from K. aerogenes for overexpression.
    Depositor
    Jennifer Doudna
    Insert
    Kae(10xHis-MBP-TEV-)CanAB
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248959-plasmid-map-sequence-id-500740

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  40. exp_KaeCanC

    Plasmid
    #248960
    Purpose
    His-MBP-CanC from K. aerogenes for overexpression.
    Depositor
    Jennifer Doudna
    Insert
    Kae(10xHis-MBP-TEV-)CanC
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    248960-plasmid-map-sequence-id-500737

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Showing: 2881 - 2920 of 26133 results