We narrowed to 9,927 results for: ADA
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Plasmid#220206PurposeMammalian expression of TEX264 with SIM2 motif mutated, C-terminal FlagDepositorInsertTEX264 (TEX264 Human)
UseTagsFlagExpressionMammalianMutationp.111ILSIW>ALAIA; c.421A>G,422T>C,427T&g…PromoterCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-TEX264-SHP1mut-cFlag
Plasmid#220208PurposeMammalian expression of TEX264 with SHP domain mutated, C-terminal FlagDepositorInsertTEX264 (TEX264 Human)
UseTagsFlagExpressionMammalianMutationp.280GEGPL>RERPA; c.838G>C,844G>A,850T&g…PromoterCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-TEX264-SIM2_mut-cGFP
Plasmid#220213PurposeMammalian expression of TEX264 with SIM2 motif mutated, fused at C-terminus to GFPDepositorInsertTEX264 (TEX264 Human)
UseTagsGFPExpressionMammalianMutationp.111ILSIW>ALAIA; c.421A>G,422T>C,427T&g…PromoterCMV/TetOAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/666E
Plasmid#105313PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159/666EPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP KInd2 S159E
Plasmid#105311PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159EPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/666A
Plasmid#105309PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159/666APromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S666A
Plasmid#105308PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS666APromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP KInd2 S159A
Plasmid#105306PurposeTo study altered downstream functions of phospholylation-site null kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159APromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-LIN-29-pcDNA3.1-
Plasmid#52515Purposeexpresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertLin-29 b (lin-29 Nematode)
UseTagsRGS-6xHisExpressionMammalianMutationwt (changed coding sequence in 5' (5th codon…PromoterCMVAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
miABE
Plasmid#205412PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoterDepositorInsertpU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
UseTagsExpressionMammalianMutationD61A+E85R+H369R+S387R+S457RPromoterhU6, CAG, CMVAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
loxP-G418-LoxP-TurboID-Rab11 HR
Plasmid#230026PurposeHomology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cellsDepositorInsertgenomic sequence Rab11 locus (RAB11A Human)
UseCRISPRTagsTurbo-ID, V5ExpressionMutationPromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMIG-FLAG-MLL-AF9
Plasmid#71443PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFPDepositorInsertMLL-Af9 (KMT2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
3xFlag-eGFP-Flag-SETX
Plasmid#218838PurposeMammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.DepositorInsertSenataxin (SETX Human)
UseTagsFlag and eGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
CamKII-LGI1-pHmScarlet
Plasmid#185538PurposeExpresses LGI1-pHmScarlet under the CamKII promoterDepositorInsertLGI1 (Lgi1 Rat)
UseLentiviralTagspHmScarletExpressionMammalianMutationPromoterCamKIIAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
CamKII-LGI1-pHluorin
Plasmid#185537PurposeExpresses LGI1-pHluorin under the CamKII promoterDepositorInsertLGI1 (Lgi1 Rat)
UseLentiviralTagspHluorinExpressionMammalianMutationPromoterCamKIIAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
PEmax_AAVS1_knockin_HDR_donor
Plasmid#223000PurposeHDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPEmax
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIR-FLAG-MLL-AF9
Plasmid#71444PurposeRetroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2DepositorInsertMLL-AF9 (KMT2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterMSCVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTNwt-FL
Plasmid#110217PurposeExpression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
UseTagsExpressionBacterialMutationP296L (see depositor comments below)PromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
α‐eGFP
Plasmid#160214PurposeRetroviral construct to express an eGFP-tagged version of the α subunit of AP2DepositorUseRetroviralTagseGFP at Hinge regionExpressionMutationBrain-specific InsertPromoterAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424TagsExpressionMutationThe conserved GDD in the NS5 polymerase gene muta…PromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only