We narrowed to 1,931 results for: cas9 expression vector

1. MKC86_HBA1(tEPOR-2A-YFP)

   Plasmid

   #232405

   Purpose

   AAV production plasmid for tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at HBA1 locus using HBA1-sg4 gRNA. HBA1 UTRs flank full tEPOR-2A-YFP cassette. Homology arms mediate full HBA1 replacement.

   Depositor

   Matthew Porteus, Kyle Cromer

   Article

   Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552

   Inserts

   Truncated Erythropoietin Receptor (EPOR Human)
   Hemoglobin Subunit Alpha 1 (HBA1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONOR-MARINA

   Plasmid

   #223328

   Purpose

   DONOR vector for genomic integration of MARINA voltage sensitive indicator gene from Platisa et al., 2017 (10.1021/acschemneuro.6b00234).

   Depositor

   Jonathan Arias

   Article

   Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

   Inserts

   MARINA
   KIR2.1 channel Golgi-to-plasma membrane trafficking signal
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mCherry and super-ecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MDM4_Deletion_Upstream_gRNA_2

   Plasmid

   #195135

   Purpose

   gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 Deletion Upstream gRNA 2 (MDM4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MDM4_Deletion_Upstream_gRNA_1

   Plasmid

   #195134

   Purpose

   gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 Deletion Upstream gRNA 1 (MDM4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXPR_118

   Plasmid

   #113667

   Purpose

   Lentiviral vector expressing dCas9

   Depositor

   John Doench

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   catalytically inactive

   Promoter

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

   Plasmid

   #216732

   Purpose

   Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   sgCTG lentiviral guide RNA with eGFP tag
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry_gRNA

   Plasmid

   #80457

   Purpose

   gRNA expression vector with mCherry transfection control

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Inserts

   sp Cas9 gRNA
   mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and chicken β-actin promoter

   Available since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX459-sgRNA048_Grb10

   Plasmid

   #232934

   Purpose

   Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Cas9-T2A-PuroR (Grb10 S. pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX459-sgRNA047_Podxl

   Plasmid

   #232932

   Purpose

   Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Cas9-T2A-PuroR (Podxl S. pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGTag Hygro EGFP cLAP +2

    Plasmid

    #194310

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

    Insert

    cLap(EGFP)-P2A-Hygro
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGTag Hygro EGFP cLAP +0

    Plasmid

    #194308

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

    Insert

    cLap(EGFP)-P2A-Hygro
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGTag Hygro EGFP cLAP -2

    Plasmid

    #194313

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

    Insert

    cLap(EGFP)-P2A-Hygro
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGTag Hygro EGFP cLAP -0

    Plasmid

    #194311

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

    Insert

    cLap(EGFP)-P2A-Hygro
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGTag Hygro EGFP cLAP +1

    Plasmid

    #194309

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

    Insert

    cLap(EGFP)-P2A-Hygro
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGTag Hygro EGFP cLAP -1

    Plasmid

    #194312

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

    Insert

    cLap(EGFP)-P2A-Hygro
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP

    Plasmid

    #113158

    Purpose

    An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP

    Plasmid

    #113156

    Purpose

    An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat Rosa26
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

    Plasmid

    #113159

    Purpose

    An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    gRNA for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

    Plasmid

    #113155

    Purpose

    An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat TH
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

    Plasmid

    #113157

    Purpose

    An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    Two gRNAs for rat MANF
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 and hU6

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge