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We narrowed to 25,285 results for: crispr

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Showing: 1441 - 1460 of 25285 results

  4. pKMW123_SpRY-Cas9_U6-entry

    Plasmid
    #244820
    Purpose
    Mammalian expression of SpRY Cas9 with Golden Gate-compatible sgRNA spacer cassette
    Depositor
    Jennifer Doudna
    Publication
    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpRY Cas9
    Use
    CRISPR
    Tags
    3xFLAG-SV40 NLS and Nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…
    Promoter
    CAG, U6
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244820-plasmid-map-sequence-id-486582

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  8. pKMW281_dSpRY

    Plasmid
    #244822
    Purpose
    Mammalian expression of catalytically inactive SpRY Cas9 (dSpRY)
    Depositor
    Jennifer Doudna
    Publication
    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.
    Insert
    dSpRY Cas9
    Use
    CRISPR
    Tags
    3xFLAG-SV40 NLS and Nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CAG
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    244822-plasmid-map-sequence-id-486583

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  9. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Publication
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

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  10. pBTK601

    Plasmid
    #110607
    Purpose
    BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)
    Depositor
    Jeffrey Barrick
    Publication
    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    Synthetic Biology
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    110607-plasmid-map-sequence-id-215544

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  13. pWS082

    Plasmid
    #90516
    Purpose
    sgRNA entry vector
    Depositor
    Tom Ellis
    Insert
    Yeast sgRNA expression vector
    Use
    CRISPR; Yeast gap repair
    Expression
    Yeast
    Available since
    Oct. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    90516-plasmid-map-sequence-id-197186

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  16. pCut_GFP

    Plasmid
    #240154
    Purpose
    Expresses CRISPR guides
    Depositor
    Morten Norholm
    Publication
    Gude et al bioRxiv 2025.06.19.660532
    Inserts
    constant cut sites CRISPR guide array
    "self-curing" CRISPR guide array
    GFP
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    Oct. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240154-plasmid-map-sequence-id-479867

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  18. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Publication
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  19. pEJS581-pCSDest2-AcrIIC4Hpa-FLAG-NLS

    Plasmid
    #113436
    Purpose
    Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from H. parainfluenzae in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    type II-C anti-CRISPR
    Use
    CRISPR
    Tags
    FLAG/NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    113436-plasmid-map-sequence-id-225078

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  20. pTK Cas9-2A-Citrine

    Plasmid
    #92393
    Purpose
    Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9 2A Citrine
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    thymidine kinase promoter
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92393-plasmid-map-sequence-id-190866

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Showing: 1441 - 1460 of 25285 results