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Showing: 1441 - 1460 of 22521 results

  6. pGW_RUBY_EggCas9

    Plasmid
    #225990
    Purpose
    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225990-plasmid-map-sequence-id-448019

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  7. pX601 miniCMV-SaCas9-SpA-sgRNA scaffold

    Plasmid
    #107055
    Purpose
    Backbone vector for cloning in target sgRNA for use with SaCas9 (SauCas9)
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107055-plasmid-map-sequence-id-208591

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  8. pJJR81

    Plasmid
    #75029
    Purpose
    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Mike Boxem
    Insert
    tagBFP2^SEC^3xFlag
    Use
    CRISPR
    Tags
    3xFLAG and C. elegans codon optimized tagBFP2
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    75029-plasmid-map-sequence-id-141917

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  9. XClone_Cas9_KRAB_T2A_BFP

    Plasmid
    #141208
    Purpose
    Tunable and temporal expression control of dCas9
    Depositor
    Rory Johnson
    Insert
    dCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    141208-plasmid-map-sequence-id-308770

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  10. pFETCh_ZNF3

    Plasmid
    #72362
    Purpose
    Donor vector for 3' FLAG tag of human ZNF3
    Depositor
    Eric Mendenhall, Richard M. Myers
    Insert
    ZNF3 Homology Arms (ZNF3 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    72362-plasmid-map-sequence-id-132112

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  11. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid
    #221537
    Purpose
    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221537-plasmid-map-sequence-id-437448

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  12. 1203T-CMV-Cas7.1-NLS-bGHpolyA

    Plasmid
    #221540
    Purpose
    Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221540-plasmid-map-sequence-id-437823

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  13. 1203W-CMV-Cas7.1-7.2-bGHpolyA

    Plasmid
    #220961
    Purpose
    Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and Cas7.2 domains from DiCas7-11, deactivated (truncated from DiCas7-11 effector)
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    220961-plasmid-map-sequence-id-437427

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  14. pR6K-GFP-CASTIB

    Plasmid
    #199655
    Purpose
    R6K plasmid with a I-B CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST I-B systems
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199655-plasmid-map-sequence-id-412259

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  15. pR6K-GFP-CASTV

    Plasmid
    #199657
    Purpose
    R6K plasmid encoding a type V CAST but no CRISPR array
    Depositor
    IIya Finkelstein
    Insert
    CAST V systems
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    199657-plasmid-map-sequence-id-412267

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  16. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160230-plasmid-map-sequence-id-314765

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  18. lentiCas9n(D10A)-Blast

    Plasmid
    #63593
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Blasticidin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, nickase mutant
    Promoter
    EFS-NS
    Available since
    April 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63593-plasmid-map-sequence-id-181681

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  19. pRGEB32

    Plasmid
    #63142
    Purpose
    Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for Agrobacterium-mediated transformation.
    Depositor
    Yinong Yang
    Article
    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Rice snoRNA U3 promoter for gRNA/PTG expression a…
    Available since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63142-plasmid-map-sequence-id-339931

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  20. pEcCas

    Plasmid
    #73227
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgRNA
    Depositor
    Sheng Yang
    Article
    Li et al Acta Biochim Biophys Sin (Shanghai). 202
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR; Red recombinase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    native promoter
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73227-plasmid-map-sequence-id-419024

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Showing: 1441 - 1460 of 22521 results