164,515 results

1. IKK-2 S177E S181E

   Plasmid

   #11105

   Purpose

   Mammalian expression of constitutively active human IKK-2

   Depositor

   Anjana Rao

   Article

   Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

   Insert

   IKK-2 (IKBKB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S177E and S181E, constitutively active

   Promoter

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-fDIO-Cre (AAV1)

   Viral Prep

   #121675-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Alexander Nectow, Esteban Engel

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Promoter

   EF1a

   Tags

   None

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

3. pCCL/IFNB1-d2eGFP-3’UTR

   Plasmid

   #180232

   Purpose

   Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

   Insert

   d2eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   1 kb upstream of IFNB1 CDS

   Available since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnCS_SEPT6_SEPT7-TEV-Strep

   Plasmid

   #174499

   Purpose

   bacterial co-expression of human SEPT6 and of human SEPT7

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN6 (SEPTIN6 Human)
   SEPTIN7 (SEPTIN7 Human)
   

   Use

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. MS2_GFP

   Plasmid

   #61764

   Purpose

   Expresses MS2 coat protein fused to GFP

   Depositor

   Daniel Larson

   Article

   Coulon et al Elife. 2014 Oct 1;3. doi: 10.7554/eLife.

   Insert

   MS2 coat protein fused to eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc promoter

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZac2.1-GfaABC1D-lck-jGCaMP8s (AAV1)

   Viral Prep

   #176761-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8s (#176761). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8s plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8s. These AAV preparations are suitable purity for injection into animals.n into animals.

   Depositor

   Loren Looger

   Promoter

   GfaABC1D

   Tags

   None

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

7. sgRNA(MS2) cloning backbone

   Plasmid

   #61424

   Purpose

   sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. 15x-QUAS-dTomato-T2A-GCaMP6s

   Plasmid

   #130666

   Purpose

   QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   15xQUAS-dTomato-T2A-GCaMP6s
   

   Use

   Ae. aegypti expression

   Tags

   Expression

   Insect

   Mutation

   Promoter

   15xQUAS-TATA

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti X1 Puro DEST (694-6)

   Plasmid

   #17297

   Purpose

   3rd gen lentiviral promoter-less Gateway destination vector, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-ERKKTRClover

    Plasmid

    #59138

    Purpose

    ORF encoding ERK KTR mClover flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter (MAPK1 Mouse, Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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