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We narrowed to 10,558 results for: SEC

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Showing: 1461 - 1480 of 10558 results

  1. pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169183
    Purpose
    Baculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-3xFlag/nsp7-His6-nsp8 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag (nsp12 C-terminus) and His6 (as internal t…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169183-plasmid-map-sequence-id-333983

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  2. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid
    #169164
    Purpose
    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells
    Depositor
    John Diffley
    Article
    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464
    Insert
    nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    6His-3xFlag (nsp10 C-terminus)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169164-plasmid-map-sequence-id-333982

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  3. pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid
    #169181
    Purpose
    Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    No purification tag and nsp7-nsp8 fusion protein …
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169181-plasmid-map-sequence-id-334012

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  14. AAV phSyn1(S)-FlpO-bGHpA (AAV Retrograde)

    Viral Prep
    #51669-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from AAV phSyn1(S)-FlpO-bGHpA (#51669). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-FlpO-bGHpA plasmid DNA. hSyn-driven FlpO expression. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Hongkui Zeng
    Article
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Promoter
    Syn
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only

  15. pLenti-rInsp-hIns-GLuc

    Plasmid
    #89927
    Purpose
    Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    410bp rat insulin II promoter
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    89927-plasmid-map-sequence-id-255126

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  16. pcDNA3.1+(rInsp)-Ins-GLuc

    Plasmid
    #89928
    Purpose
    Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    rat Insulin II promoter
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89928-plasmid-map-sequence-id-255702

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  17. pcDNA3.1+CMV-Ins-GLuc

    Plasmid
    #89929
    Purpose
    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Human, Synthetic)
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    CMV
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89929-plasmid-map-sequence-id-255704

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  18. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid
    #169177
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells
    Depositor
    John Diffley
    Article
    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515
    Insert
    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag-His6 (nsp5-cleavable)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169177-plasmid-map-sequence-id-333223

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  19. pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)

    Plasmid
    #169194
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells
    Depositor
    John Diffley
    Article
    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531
    Insert
    3xFlag-His6-PLpro (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    3xFlag-His6
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169194-plasmid-map-sequence-id-333980

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  20. pcDNA3.1-GSDMD_1-270

    Plasmid
    #218229
    Purpose
    Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    GSDMD (GSDMD Human)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    construct only codes for amino acids 1-270
    Available Since
    July 1, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    218229-plasmid-map-sequence-id-430564

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Showing: 1461 - 1480 of 10558 results