We narrowed to 5,507 results for: SPI

1. pcDNA3.3_SARS2_BA.2.87.1

   Plasmid

   #218679

   Purpose

   expressing SARS-CoV-2 BA.2.87.1 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Insert

   Spike (BA.2.87.1)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MERS_NSP13_BV

   Plasmid

   #234378

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp13_MERS
   

   Use

   Baculovirus expresssion

   Tags

   MHHHHHHSSGRENLYFQG

   Expression

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. hIgG1-F1-Fc

   Plasmid

   #194597

   Purpose

   Mammalian expression of F1 nanobody upstream of human IgG1; F1 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Ben Murrell

   Insert

   F1-Fc (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. hIgG1-E2-Fc

   Plasmid

   #194595

   Purpose

   Mammalian expression of E2 nanobody upstream of human IgG1; E2 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Ben Murrell

   Insert

   E2-Fc (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. hIgG1-G6-Fc

   Plasmid

   #194594

   Purpose

   Mammalian expression of G6 nanobody upstream of human IgG1; G6 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Ben Murrell

   Insert

   G6-Fc (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. hIgG1-C7-Fc

   Plasmid

   #194596

   Purpose

   Mammalian expression of C7 nanobody upstream of human IgG1; C7 nanobody targets the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Ben Murrell

   Insert

   C7-Fc (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SARS2nsp3_MacroD_ECBio2

   Plasmid

   #234388

   Purpose

   Bacterial expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp3_SARS2
   

   Use

   Tags

   LVPRGSSSKGGYGLNDIFEAQKIEWHE and MHHHHHHSSGVDLGTEN…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. nsp14_EC_Bio1

   Plasmid

   #234376

   Purpose

   Bacterial expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp14_SARS2
   

   Use

   Tags

   GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SARS2nsp3_MacroD_EC

   Plasmid

   #234389

   Purpose

   Bacterial expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   Nsp3_SARS2
   

   Use

   Tags

   MHHHHHHSSGVDLGTENLYFQSM

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. phCMV-229E-D15

    Plasmid

    #188908

    Purpose

    express truncated 229E spike for pseudovirus production

    Depositor

    David Nemazee

    Insert

    229E spike (S 229E)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c-terminal 15aa deletion

    Promoter

    CMV

    Available since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.3_SARS2_XBB.1.5

    Plasmid

    #196585

    Purpose

    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (XBB.1.5)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G252V, F486P on the basis of XBB

    Promoter

    CMV

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

    Plasmid

    #160629

    Purpose

    TU for the expression of LbCas12a

    Depositor

    Diego Orzaez

    Insert

    AtUBQ10-:LbCpf1(Cas12a):Tnos
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    UBQ10

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET24a MDH_AQUAR

    Plasmid

    #204239

    Purpose

    Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_AQUAR
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p112A1NE

    Plasmid

    #85983

    Purpose

    For construction of cDNA expression library.

    Depositor

    Wolf Frommer

    Article

    Riesmeier et al EMBO J. 1992 Dec;11(13):4705-13.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Adh1

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-EgC*

    Plasmid

    #20378

    Depositor

    Andrew Lee

    Article

    Hu et al Biochemistry. 2003 Dec 2. 42(47):13856-6

    Insert

    Eglin C wt*
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F10W

    Promoter

    Available since

    April 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAIP4_6WEY

    Plasmid

    #234234

    Purpose

    Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pAIP4_5HIH

    Plasmid

    #234205

    Purpose

    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pAIP4_2H2Z

    Plasmid

    #234147

    Purpose

    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. mEGFP

    Plasmid

    #18696

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    monomeric EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. paGFP

    Plasmid

    #18697

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    photoactivatable GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K mutation

    Promoter

    CMV

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge