Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,441 results for: dcas9

Clear keywords
Showing: 1461 - 1480 of 2441 results

  2. pCXLE-dCas9VPH-T2A-GFP-shP53

    Plasmid
    #102895
    Purpose
    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9VPH-T2A-GFP-shP53
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102895-plasmid-map-sequence-id-194733

    Enlarge

  4. XClone_Cas9_KRAB_T2A_BFP

    Plasmid
    #141208
    Purpose
    Tunable and temporal expression control of dCas9
    Depositor
    Rory Johnson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Expression
    Mammalian
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    141208-plasmid-map-sequence-id-308770

    Enlarge

  6. pJT242 Lenti pEF-dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast

    Plasmid
    #187327
    Purpose
    dCas9 effector fusion for manipulating transcription
    Depositor
    Michael Bassik, Lacra Bintu
    Publication
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    dCas9-3XFLAG-ZNF10 KRAB[WSR7EEE] HighExpr-T2A-tagBFP-P2A-Blast
    Use
    CRISPR and Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    KRAB WSR7EEE
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187327-plasmid-map-sequence-id-373284

    Enlarge

  8. pCENPTΔC-dCas9-3xGFP

    Plasmid
    #198325
    Purpose
    Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus
    Depositor
    Sarah McClelland
    Publication
    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:
    Insert
    CENPTΔC (CENPT Human)
    Use
    Lentiviral
    Tags
    EGFP x 3 and dCas9
    Expression
    Mammalian
    Mutation
    truncation - encodes only amino acids 1-375
    Promoter
    CMV-TetO
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198325-plasmid-map-sequence-id-390299

    Enlarge

  10. pPPC010.AAV

    Plasmid
    #171175
    Purpose
    Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid
    Depositor
    Jesse Zalatan
    Publication
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Inserts
    hAAVS1 scRNA
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    BBa_J23119 and Sp.pCas9, BBa_J23107
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171175-plasmid-map-sequence-id-344748

    Enlarge

  12. dCas9-GCN5 core

    Plasmid
    #179551
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179551-plasmid-map-sequence-id-354777

    Enlarge

  13. pdCas9-GFP-p65

    Plasmid
    #232630
    Purpose
    p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer
    Depositor
    Karsten Rippe
    Insert
    dCas9-GFP-p65 (RELA Synthetic, Human)
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232630-plasmid-map-sequence-id-463624

    Enlarge

  16. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187957
    Purpose
    FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-KRAB-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187957-plasmid-map-sequence-id-373706

    Enlarge

  19. pLY305

    Plasmid
    #184159
    Purpose
    Reporter circuit for hijacking mRNA of zraP as CRISPR RNA
    Depositor
    Baojun Wang
    Publication
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    mutsfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    K140N, Mutations D10A and H840A on WT cas9. Synon…
    Promoter
    Pcon, PpspA-zraP-22, PrhaB, and Ptet
    Available since
    Oct. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    184159-plasmid-map-sequence-id-376083

    Enlarge

Showing: 1461 - 1480 of 2441 results