We narrowed to 1,854 results for: rigi

1. pTC3cIfm

   Plasmid

   #193778

   Purpose

   Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   Frame-shifted CI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLTetO-1

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HyWnt3-ΔRep-D1:Luc

   Plasmid

   #192988

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D1)

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial deletion of the Wnt3 repressor element-D1

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HyWnt3-ΔRep-D2:Luc

   Plasmid

   #192989

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D2)

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial deletion of the Wnt3 repressor element-D2

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HyWnt3-ΔRep-D4:Luc

   Plasmid

   #192991

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity (partial deletion of the repressor element-D4)

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial deletion of the Wnt3 repressor element-D4

   Promoter

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HyWnt3-ΔRep:Luc

   Plasmid

   #192987

   Purpose

   Plasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element)

   Depositor

   Brigitte Galliot

   Article

   Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

   Insert

   Hydra Wnt3 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Repressor element deleted

   Promoter

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQmod3S-GG

   Plasmid

   #191349

   Purpose

   Clostridium expression vector (pCB102 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Insert

   Plac-RFP (IPTG-inducible red fluorescent protein)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. QUAS-0-T7-TetO-GFP_T7-LacO-TetR_ColE1

   Plasmid

   #171663

   Purpose

   QUAS directly upstream of a T7 promoter with a TetO operator. Expression of TetR in BL21(DE3) E. coli. GFP is expressed according to presence of aTc. Contains the ColE1 origin of replication.

   Depositor

   Tara Deans

   Article

   MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

   Insert

   QUAS-T7-TetO-GFP ssrA-T7 Stop- T7-LacO-TetR-T7 Stop
   

   Use

   Tags

   ssrA degradation tag (DAS+4)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pR6Kg-Alpha2

   Plasmid

   #165863

   Purpose

   Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RIB ONLY R3

   Plasmid

   #161511

   Purpose

   Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R3_gRNA_MS2

   Depositor

   Brigitte Gasser, Diethard Mattanovich

   Article

   Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

   Inserts

   dCAS9
   MS2 (non activation control NAC)
   gRNA (Targeting R3 in the RIB1 promoter)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGAP, pPOR1, and pTEF2

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RIB ONLY R4

    Plasmid

    #161512

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_RIB1_R4_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting R4 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. THI ONLY pALD4 T1

    Plasmid

    #161498

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T1_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T1 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. THI ONLY pALD4 T2

    Plasmid

    #161499

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T2_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T2 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. THI ONLY pALD4 T3

    Plasmid

    #161500

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T3_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T3 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. THI ONLY pALD4 T4

    Plasmid

    #161501

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T4_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T4 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. THI ONLY pALD4 T5

    Plasmid

    #161502

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THI11_T5_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T5 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. THI ONLY pGAP T-

    Plasmid

    #161503

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T-_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. THI ONLY pGAP T1

    Plasmid

    #161504

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T1_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T1 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. THI ONLY pGAP T2

    Plasmid

    #161505

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T2_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T2 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. THI ONLY pGAP T3

    Plasmid

    #161506

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T3_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T3 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. THI ONLY pGAP T4

    Plasmid

    #161507

    Purpose

    Non activation control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pGAP_THI11_T4_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2 (non activation control NAC)
    gRNA (Targeting T4 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge