199,099 results

1. CRY2olig-GP

   Plasmid

   #218918

   Purpose

   Expression of CRY2olig-mCherry fused to a codon-optimised poly-GP sequence

   Depositor

   Tatyana Shelkovnikova

   Article

   Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

   Insert

   CRY2olig-mCherry tagged poly-GP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild-type

   Promoter

   CMV

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-CMV-Galpha-s ONE-GO

   Plasmid

   #189731

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-s ONE-GO
   GNAS (GNAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA5/FRT/TO-GAlphasS-RLuc8

   Plasmid

   #140980

   Purpose

   Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphasS-RLuc8
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHEE401E

   Plasmid

   #71287

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   gRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR1326

   Plasmid

   #111131

   Purpose

   Expresses UBE2T.

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   UBE2T (UBE2T Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-CMV-PRB

   Plasmid

   #206165

   Purpose

   pMVP Gateway destination vector (3rd generation lentiviral vector) containing human full length PRB gene

   Depositor

   Renee van Amerongen

   Article

   Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

   Insert

   Progesteron receptor B (PGR Human)
   

   Use

   Lentiviral; Pmvp gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT260 Lenti pEF-dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast

   Plasmid

   #187336

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-VPR-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-hPTGS2-2flag

   Plasmid

   #102498

   Purpose

   Mammalian expression of PTGS2

   Depositor

   Jun Yu

   Article

   Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

   Insert

   PTGS2 (PTGS2 Human)
   

   Use

   Tags

   Flag-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

   Plasmid

   #159630

   Purpose

   AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

   Depositor

   Ross Bathgate, Andrew Allen

   Article

   Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

   Insert

   Ast (AstA Fly)
   

   Use

   AAV

   Tags

   HA, IRES, mCherry, and neurophysin-1

   Expression

   Mammalian

   Mutation

   Promoter

   DCA (same as CAG promoter)

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIP-AMPKa1_KD

    Plasmid

    #79011

    Purpose

    For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47R

    Promoter

    pCAGGs

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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