We narrowed to 2,957 results for: BFP

1. pTS102 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOstar-MCS

   Plasmid

   #199355

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOstar-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagBFP-P2A-ALFA-SUMOstar

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS112 pHAGE2 CMVtetO2 MCS-TEV-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199365

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter)

   Plasmid

   #128609

   Purpose

   Expresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   uORF1-36xMashTag
   

   Expression

   Mammalian

   Promoter

   Pcmv

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUW-crRNA-scrambled-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

   Plasmid

   #194897

   Purpose

   Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and scrambled crRNA without target in human genome.

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Inserts

   dLbCpf1-NLS-hCTCF_WT
   U6-crRNA-scramble
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Human codon optimized catalytically inactive LbCp…

   Promoter

   TRE and U6

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199368

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-SUMOstar-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

   Plasmid

   #170183

   Purpose

   Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

   Depositor

   Hunter Fraser

   Article

   Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

   Insert

   sgBFP
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEJS2784 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-20a CMV-BFP

   Plasmid

   #240514

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Tags

   FLAG, hsa-miR-20 Target Sites, and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEJS2785 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-125a CMV-BFP

   Plasmid

   #240515

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Tags

   FLAG, hsa-miR-125a Target Sites, and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS2786 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-151a CMV-BFP

   Plasmid

   #240516

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Tags

   FLAG, hsa-miR-151a Target Sites, and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS2787 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-221 CMV-BFP

    Plasmid

    #240517

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Tags

    FLAG, hsa-miR-221 Target Sites, and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. XZ066-(PB)EF1a-LIDARST-CCR6V2-IRESv0-T2A-PuroR-P2A-BFP

    Plasmid

    #233418

    Purpose

    PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP
    

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available Since

    June 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS1386 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-107 CMV-BFP

    Plasmid

    #226115

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Tags

    FLAG, hsa-miR-107 Target Sites, and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS1350 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-92a CMV-BFP

    Plasmid

    #226109

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Tags

    FLAG, hsa-miR-92a Target Sites, and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS1351 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-378a CMV-BFP

    Plasmid

    #226110

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Tags

    FLAG, hsa-miR-378a Target Sites, and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS1353 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-33b CMV-BFP

    Plasmid

    #226112

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Tags

    FLAG, hsa-miR-33b Target Sites, and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-rev(CMV-TagBFP2)-EFS-mCherry-124-MUT-MRE

    Plasmid

    #235148

    Purpose

    Expresses the mCherry-based mutated (control) sensor for miR-124. TagBFP2 is expressed as a transduction reporter

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    mCherry with a mutated miR-124 MRE
    

    Use

    AAV

    Mutation

    7 nucleotides of the miR-124 WT MRE mutated

    Promoter

    EFS

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. LSM053 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-mtagBFP (FLP-IN)

    Plasmid

    #233409

    Purpose

    LIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, mTagBFP as output

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-SGc(stem-1(3xPP7))cSG-mtagBFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9

    Plasmid

    #231910

    Purpose

    For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid

    #202555

    Purpose

    Synaptotagmin-1 sgRNA2 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid

    #202556

    Purpose

    Synaptotagmin-1 sgRNA3 with TagBFP2

    Depositor

    Frederic Meunier

    Article

    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

    Insert

    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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