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pADH118-2

Plasmid

#90984

Purpose

NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. albicans LEU2 2 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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FCK-Arch-GFP

Plasmid

#22217

Purpose

Lentiviral expression of Arch-GFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pLPO2

Plasmid

#209976

Purpose

Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (SH71_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBC1FG

Plasmid

#31346

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Cry1 (Cry1 Mouse)

Tags

Flag and His

Expression

Insect

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pCAS1yl

Plasmid

#73226

Purpose

Constitutive expression of Cas9 and sgRNA in Yarrowia lipolytica cells

Depositor

Sheng Yang

Article

Gao et al J Ind Microbiol Biotechnol. 2016 Aug;43(

Inserts

Cas9
sgRNA

Use

CRISPR; In y. lipolytica

Expression

Bacterial

Promoter

unknown

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLPO1

Plasmid

#209975

Purpose

Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (256_rep)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCKFB1

Plasmid

#31280

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Clock (Clock Mouse)

Tags

Flag and His

Expression

Insect

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pHBV-X/EnhI-Luc

Plasmid

#71418

Purpose

To see HBV X promoter(overlaped EnhanceI) activity for luciferase assay

Depositor

Wang-Shick Ryu

Article

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV X promoter and enhancer I driving Luciferase expression

Use

Luciferase

Expression

Mammalian

Promoter

HBV X

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-13Myc-His3MX6

Plasmid

#41602

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

13Myc, A. gossypii translation elongation factor …

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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Plas-gRNA-LEU2

Plasmid

#157656

Purpose

GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

Depositor

Frank Albert

Article

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

GFP-gRNA

Use

Insert storage (replicative in e. coli)

Tags

GFP-gRNA

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-RhoC

Plasmid

#23226

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoC (RHOC Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
Plas-CRISPR_reporter

Plasmid

#157658

Purpose

CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

Depositor

Frank Albert

Article

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

dCAS9-VP64, mCherry, KanMX

Use

Insert storage (replicative in e. coli)

Mutation

N28D in mCherry- please see depositor comments be…

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-TC10

Plasmid

#23232

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

TC10 (RHOQ Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pPYL-ABI

Plasmid

#233650

Purpose

Cellular colocalization assay (PYL/ABI) for the CIP OP (opabactin) or the CIP ABA (abscisic acid)

Depositor

Richard Wombacher

Article

Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

Insert

TOMM20-mCherry-PYL-P2A-eGFP-ABI

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pDJSVT86

Plasmid

#108965

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

Depositor

Daniel Slade

Article

Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

Type

Empty backbone

Tags

OmpA 1-27, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Sirius-mT-Sapphire_pcDNA3

Plasmid

#89584

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

Sirius-mT-Sapphire

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCKPC4

Plasmid

#31366

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Clock (Clock Mouse)

Tags

Flag and His

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
pMC1SG5

Plasmid

#31282

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Cry1 (Cry1 Mouse)

Tags

Flag, His, and Myc

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only
nls-Flamindo2

Plasmid

#73939

Purpose

Expresses biosensor for cAMP in mammalian cells with nuclear localization signal

Depositor

Tetsuya Kitaguchi

Article

Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

Insert

nls-Flamindo2

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-hRhoD

Plasmid

#23235

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoD (RHOD Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only