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pHS1_10xHis_MBP_TEV_WT-Cas9

Plasmid

#244825

Purpose

Bacterial expression of SpyCas9 (WT) for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-DII-ω2

Plasmid

#242518

Purpose

Donor II vector in DASH and omega2 vector in GoldenBraid/DASH, with attPCC and attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDASH-AIK

Plasmid

#242525

Purpose

Acceptor vector I, accepting Donor I first, with attPTT-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14824: pAAV-AiE0441h_3xC2-minBG-iCre-BGHpA (Alias: CN4824)

Plasmid

#214861

Purpose

Expression of iCre in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

iCre

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Med24-exon minimal CMV pCDNA5

Plasmid

#212060

Purpose

LR vector for integration of Med24-exon into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Med24-exon (Med24 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pYS29

Plasmid

#202086

Purpose

Parent vector for producing universal minicircle donors for HaloTag N-terminal knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

NT HygR-T2A-Halo Minicircle Parent

Use

CRISPR

Tags

HaloTag

Promoter

None

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN(CMV-copGFP-Luc2-Puro)

Plasmid

#80389

Purpose

To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

Depositor

Magnus Essand

Article

Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

Insert

copGFP-T2A-Luc2-F2A-Puromycin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218857

Purpose

Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Tags

QF fusion protein

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

Plasmid

#105648

Purpose

Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks

Depositor

Sandra Rieger

Insert

Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)

Expression

Mammalian

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd

Plasmid

#80950

Purpose

Expresses Gsdmd in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd (Gsdmd Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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