164,599 results

1. pTP728

   Plasmid

   #216374

   Purpose

   xNup358-Nb1t (+3Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xNup358-Nb1t (+3Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCL758

   Plasmid

   #219504

   Purpose

   Express Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

   Insert

   msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYN2_1

   Plasmid

   #184757

   Purpose

   Plasmid for genome editing by CRISPR/Cas9

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   Cas9
   sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
   

   Use

   Synthetic Biology

   Tags

   2xNLS

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   PGK1 and tRNA-Phe-HDV Ribozyme

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. CL20_mEGFP_TMPRSS2_ETV1

   Plasmid

   #205944

   Purpose

   Express mEGFP-tagged fusion protein, TMPRSS2_ETV1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   TMPRSS2_ETV1 (TMPRSS2, ETV1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA11-pMa-sgTS3-17xMS2V5-sgTS3

   Plasmid

   #199442

   Purpose

   CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

   Depositor

   Baohui Chen

   Article

   Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

   Insert

   HITI donor template
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmiRFP720-Actin-C-18

   Plasmid

   #197221

   Purpose

   Expresses the fusion protein of miRFP720-Actin-C-18 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   miRFP720-Actin-C-18
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD649-HAsp-KIR2DL5A-COMP5AP-AviTag-9xHis

   Plasmid

   #157207

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR2DL5A (KIR2DL5A Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-VSTM2A-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156633

   Purpose

   Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   VSTM2A (VSTM2A Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCytERM-mRhubarb720-N1

   Plasmid

   #197191

   Purpose

   Expresses the fusion protein of CytERM-mRhubarb720 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   CytERM-mRhubarb720
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-N3-FLuc-3BoxB_O

    Plasmid

    #147078

    Purpose

    Mammalian Expression of 3BoxB

    Depositor

    Elisa Izaurralde

    Insert

    3BoxB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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