Addgene

We narrowed to 7,015 results for: Tet-on

Showing: 1501 - 1520 of 7015 results

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pVH265-Tier1-OTetO7-PCMVmin2-TS-SEAP

Plasmid

#169588

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression with a suppressor 5'UTR (OTetO7-PCMVmin-2-5'UTRTS-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO7-PCMVmin-2

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH255-Tier1-OTetO7-PCMVmin1-SEAP

Plasmid

#169587

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-1-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO7-PCMVmin-1

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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attB53-Pac-tetO-mCherry-attB53

Plasmid

#183612

Purpose

RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

tetO-mCherry

Use

Rmce

Tags

Expression

Mutation

Promoter

tetO

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS3-TeTLc-P2A-mCherry

Plasmid

#178708

Purpose

AAV vector for Flp-dependent transgene expression of TeTLc-P2A-mCherry in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

TeTLc-P2A-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-3exo-Tetra-com-CLCN5-sp-g1

Plasmid

#176236

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176237

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176238

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL

Plasmid

#172208

Purpose

AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1S promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

Depositor

Garrett Kaas

Article

Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

Insert

HA-TALE(HD)-Tet1S-NLS-SID4X_2A_EGFP_W3SL

Use

AAV and Mouse Targeting; Tale

Tags

HA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_HA-TALE(NG)-Tet1FL-NLS-SID4X_2A_EGFP_W3SL

Plasmid

#172205

Purpose

AAV vector expressing an HA-tagged TALE-SID4X transcriptional repressor protein targeting the mouse Tet1FL promoter. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.

Depositor

Garrett Kaas

Article

Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d

Insert

HA-TALE(NG)-Tet1FL-SID4X-NLS_2A_EGFP_W3SL

Use

AAV and Mouse Targeting; Tale

Tags

HA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-MCS-EnR_C-term

Plasmid

#136885

Purpose

Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codon

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-MCS-VP64_C-term

Plasmid

#136887

Purpose

Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the VP64 activator domain and a STOP codon

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

VP64 activator domain

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-MCS-KRAB_C-term

Plasmid

#136889

Purpose

Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the KRAB repressor domain and a STOP codon

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

KRAB repressor domain

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI733 pRPR1(TetO)-RPC31-sgRNA

Plasmid

#164913

Purpose

For yeast genomic integration of sgRNA against RPC31

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-RPC31-sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

RPR1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI732 pRPR1(TetO)-HTS1-sgRNA

Plasmid

#164912

Purpose

For yeast genomic integration of sgRNA against HTS1

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-HTS1-sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

RPR1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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TET-pLKO-neo NXPH4-sh3

Plasmid

#163746

Purpose

Lentivirus for inducible knockdown of NXPH4 (human)

Depositor

Roland Friedel

Insert

NXPH4 (NXPH4 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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TET-pLKO-neo NXPH4-sh2

Plasmid

#163745

Purpose

Lentivirus for inducible knockdown of NXPH4 (human)

Depositor

Roland Friedel

Insert

NXPH4 (NXPH4 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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TET-pLKO-neo NXPH4-sh1

Plasmid

#163744

Purpose

Lentivirus for inducible knockdown of NXPH4 (human)

Depositor

Roland Friedel

Insert

NXPH4 (NXPH4 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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KO602: pMVP (L3-L2) HA tag + pA; CMV::TETa-pA

Plasmid

#121802

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + CMV::TETa-polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KO502: pMVP (L3-L2) HA tag + pA; RIP::TETa-pA

Plasmid

#121810

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + RIP::TETa-polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-TET1-746-951(pL72)

Plasmid

#124406

Purpose

TET1-746-951, containing SID, used for Co-IP.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

FLAG-TET1-746-951 (TET1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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