We narrowed to 6,913 results for: dna plasmids

1. pSpCas9_T2A_mCherry sgBFP

   Plasmid

   #192929

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-SYN-PIK3CD

   Plasmid

   #203730

   Purpose

   AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Insert

   PIK3CD (PIK3CD Human)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human synapsin promoter

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BL21 Rosetta2 ΔrecBCD

   Bacterial Strain

   #176583

   Purpose

   E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

   Depositor

   Jean-Loup Faulon

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Bacterial Resistance

   Chloramphenicol

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

4. BL21 Rosetta2 ΔrecB

   Bacterial Strain

   #176582

   Purpose

   E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

   Depositor

   Jean-Loup Faulon

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Bacterial Resistance

   Chloramphenicol

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

5. pCW57-MCS1-P2A-MCS2 (Neo)

   Plasmid

   #89180

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLSLGUS

   Plasmid

   #51517

   Purpose

   pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   GUS
   3kb filler sequence
   

   Use

   Plant t-dna plasmid

   Tags

   NLS and flag

   Promoter

   virion-sense LIR and 2x35S

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLSLZ.D

   Plasmid

   #51496

   Purpose

   pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   Zif268:FokI
   us:NPTII repair template
   

   Use

   Plant t-dna plasmid

   Tags

   SV40 NLS

   Promoter

   virion-sense LIR and 2x35S

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAH121

   Plasmid

   #164901

   Purpose

   The pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.

   Depositor

   Keith Shearwin

   Article

   Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612

   Insert

   phi21 Integrase
   

   Expression

   Bacterial

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4092677

   Plasmid

   #153883

   Purpose

   Bacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to orf1b stop

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   fragment SARS-CoV-2 orf1a stop to orf1b stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Bacterial

   Mutation

   wt;handful of SNPs as watermarks

   Promoter

   Promoter | t7 consensus from NEB, G is +1

   Available Since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pSG7084 pG46-46

    Plasmid

    #232621

    Purpose

    pG46 derivative containing two replacement cassette sites (siteA and siteB) for gap-replacement-based chemical modification(s).

    Depositor

    Stephan Gruber

    Type

    Empty backbone

    Use

    Precursor plasmid to generate modified dna circles

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-sCAG-eGFP-ACAP1

    Plasmid

    #203800

    Purpose

    AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    ACAP1 (ACAP1 Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-sCAG-eGFP-RAB11

    Plasmid

    #203799

    Purpose

    AAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-SYN-TdTomato-RAB11

    Plasmid

    #203732

    Purpose

    AAV vector plasmid expressing human RAB11 fused to TdTomato under the human synapsin (SYN) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    TdTomato

    Expression

    Mammalian

    Promoter

    Human synapsin promoter

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-SYN-eGFP-RAB11

    Plasmid

    #203731

    Purpose

    AAV vector plasmid expressing human RAB11 fused to eGFP under the human synapsin (SYN) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    Human synapsin promoter

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQL269

    Plasmid

    #59802

    Purpose

    The univector plasmid-fusion system (UPS) uses Cre– lox site-specific recombination to fuse a plasmid containing the gene of interest and host vectors containing regulatory information.

    Depositor

    Stephen Elledge

    Article

    Liu et al Curr Biol. 1998 Dec 3;8(24):1300-9.

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Bacterial

    Promoter

    lambdaPR

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pUPD2

    Plasmid

    #68161

    Purpose

    Universal domesticator plasmid for level 0 parts

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLSLZ

    Plasmid

    #51495

    Purpose

    pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    Zif268:FokI
    3kb filler sequence
    

    Use

    Plant t-dna plasmid

    Tags

    SV40 NLS

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRL528

    Plasmid

    #58495

    Purpose

    helper plasmid for bacterial conjugal DNA transfer

    Depositor

    Peter Wolk

    Article

    Elhai et al Methods Enzymol. 1988;167:747-54.

    Insert

    encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    

    Available Since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGM190

    Plasmid

    #69614

    Purpose

    inducible gene expression in Streptomyces

    Depositor

    Günther Muth

    Article

    Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

    Inserts

    aphII
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Streptomyces- e. coli shuttle vector

    Expression

    Bacterial

    Available Since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBIS-GALkFLP(URA)

    Plasmid

    #49460

    Purpose

    overexpression of Flp step-arrest mutant in yeast, URA selection

    Depositor

    Marc Gartenberg

    Article

    Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

    Insert

    Flp(H305L) (flp Budding Yeast)
    

    Expression

    Yeast

    Mutation

    H305L, ‘step-arrest’ mutant,

    Promoter

    GAL10

    Available Since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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