We narrowed to 18,708 results for: met

1. pNSSCP

   Plasmid

   #183188

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   DivIVA and superfolder GFP fusion protein
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI

   Plasmid

   #84257

   Purpose

   Expresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulation

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9-ABI
   GID1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBABE-LynG

   Plasmid

   #206867

   Purpose

   Expression of LynG probe - LynAnchor-eGFP

   Depositor

   Dirk-Peter Herten

   Article

   Tashev et al Nat Methods. 2024 Sep;21(9):1708-1715. d

   Insert

   LynG
   

   Use

   Retroviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Contains Lyn amino acids 1-16

   Promoter

   Available Since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1-dCas9-MQ1(C141S)-EGFP

   Plasmid

   #89635

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG, 6*His, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   C141S, TGC-->TCC

   Promoter

   CMV

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS313_ATP3T911A

   Plasmid

   #120254

   Purpose

   Expression of ATP3-6 in yeast

   Depositor

   Markus Ralser

   Article

   Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

   Insert

   ATP3 (ATP3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T911 changed to A; Isoleucine 304 changed to Aspa…

   Promoter

   ATP3

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCIneo-lambdaN-HA-HsDCP1a_G

   Plasmid

   #146400

   Purpose

   Mammalian Expression of HsDcp1a

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

   Insert

   HsDcp1a (DCP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations T237C and G1716T compared to…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pF711-pET28a-Hs-delta25-MCAD-NHis wt

   Plasmid

   #85112

   Purpose

   expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta25) MCAD (ACADM Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCCL-PGK-SPdCas9-BFP-EGFP

   Plasmid

   #66821

   Purpose

   dCas9 fused to BFP and EGFP

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-EGFP
   

   Use

   Tags

   dSpCas9-BFP-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3b(1454/3172)P2P-wt

   Plasmid

   #21404

   Depositor

   Eric Metzen

   Article

   Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

   Insert

   EGLN1 (EGLN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pdest dTomato Puro

    Plasmid

    #223054

    Purpose

    dTomato gene expression in mammalian cells

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    dTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. rag2:CTDSP2_CE_pISceI

    Plasmid

    #223033

    Purpose

    Express CTDSP2 gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    CTDSP2
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJRoC30_Th3.1

    Plasmid

    #188065

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.1)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Use

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 4 FuncLib mutations, described in publ…

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJRoC30_Th3.7

    Plasmid

    #188066

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.7)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Use

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 3 FuncLib mutations, described in publ…

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJRoC30_Th3.10

    Plasmid

    #188067

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.10)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Use

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 3 FuncLib mutations, described in publ…

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSCrhaB2_ApyOAHIDS

    Plasmid

    #226246

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    ApyO
    ApyH
    ApyI
    ApyS
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-Synapsin-SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC

    Plasmid

    #213535

    Purpose

    Expresses the split transcription factors in cultured neuron and mouse brain

    Depositor

    Wenjing Wang

    Article

    Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

    Insert

    SspB-EGFP-VP16-P2A-FLAG-TetR-NLS-CapN-SsrA-CapC-HA-HA
    

    Use

    AAV

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKG-GW2-NgBR

    Plasmid

    #203165

    Purpose

    Yeast expression vector for hNUS1

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    NUS1 (NUS1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. DNMT2Δ191-237

    Plasmid

    #198382

    Purpose

    Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

    Depositor

    Tanja Schirmeister

    Article

    Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

    Insert

    DNMT2 (TRDMT1 Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 191-237

    Promoter

    T7

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p426-Cup1p-FusionRed

    Plasmid

    #188392

    Purpose

    Cu2+-dependent expression of red fluorescent protein in yeast cells

    Depositor

    Michihiko Kataoka

    Article

    Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

    Insert

    FusionRed
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1 promoter

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSFDuetG-HsDCP1a_539-582 (MCSII)_G

    Plasmid

    #146407

    Purpose

    Bacterial Expression of HsDCP1a_539-582

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    HsDCP1a_539-582 (DCP1A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one silent mutation T1716G compared to the sequen…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge