166,779 results

1. pSG 25

   Plasmid

   #63867

   Purpose

   Expression of beta-galactosidase at high levels in E. coli

   Depositor

   Albert Dahlberg

   Article

   O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

   Insert

   lacZ (lacZ Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PtacI

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

2. hPGK-rtTA3

   Plasmid

   #195883

   Purpose

   Lentiviral construct of hPGK driven rtTA3

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   rtTA3
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-MAST1

   Plasmid

   #23855

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   MAST1 (MAST1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pFA6a-5FLAG-KanMX6

   Plasmid

   #15983

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Type

   Empty backbone

   Use

   Tags

   5xFlag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. Halo-Sec23A

   Plasmid

   #166892

   Purpose

   mammalian expression of Sec23A tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   SEC23A (SEC23A Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. UbcH5A

   Plasmid

   #61081

   Purpose

   express a human E2

   Depositor

   Cynthia Wolberger

   Article

   Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

   Insert

   UbcH5A (UBE2D1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pAAV_hSyn1-SIO-stGtACR1-FusionRed

   Plasmid

   #105678

   Purpose

   Expresses a soma-targeted GtACR1 in Cre-expressing neurons

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

   Has Service

   AAV5

   Insert

   stGtACR1
   

   Use

   AAV

   Tags

   FusionRed and Kv2.1

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available Since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-m-Paxillin

   Plasmid

   #80023

   Purpose

   Mammalian expression of mouse EGFP-Paxillin

   Depositor

   Chinten James Lim

   Insert

   Paxillin (Pxn Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDJSVT87

   Plasmid

   #109302

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and His6 tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

   Type

   Empty backbone

   Use

   Tags

   OmpA 1-27, 6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABE HA Chk2 KD, dominant negative chk2

    Plasmid

    #41902

    Depositor

    Stephen Elledge

    Article

    Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

    Insert

    Chk2 (CHEK2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D347A

    Promoter

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA

    Plasmid

    #107313

    Purpose

    Encoding aCamkII-mCherry-Cre

    Depositor

    Hui Yang

    Article

    Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

    Insert

    aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS SK mCherryROSAegfp

    Plasmid

    #54322

    Purpose

    Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and EGFP
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWG_RUBYseed

    Plasmid

    #225989

    Purpose

    To clone any gene of interest using gateway recombination cloning and the vector expresses seed-specific RUBY

    Depositor

    Imran Siddiqi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Oct. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid

    #223127

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Syn_CapChR2_mScarlet

    Plasmid

    #188039

    Purpose

    Expresses CapChR2-mScarlet in mammalian neurons

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-mScarlet
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    hSyn

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBX_RPS2_smGFP_V5

    Plasmid

    #236088

    Purpose

    Constitutively encodes RPS2-smGFP-V5 (sfGFP-based spaghetti monster fluorescent protein embedding V5 epitope tags), for targeting the 40S subunit.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    RPS2-smGFP-V5 (RPS2 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.hSyn.CaMPARI.WPRE.SV40 (AAV1)

    Viral Prep

    #100832-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.hSyn.CaMPARI.WPRE.SV40 (#100832). In addition to the viral particles, you will also receive purified pAAV.hSyn.CaMPARI.WPRE.SV40 plasmid DNA. Synapsin-driven, photoconvertible (irreversible green-to-red) calcium integrator. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger, Eric Schreiter

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Promoter

    Syn

    Tags

    None

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pDONR221_SLC25A28

    Plasmid

    #132016

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A28 (SLC25A28 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMK465 (mAB-Hygro)

    Plasmid

    #214391

    Purpose

    mAID-BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    mAID-BrpmpTag-Hygro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMG046 pETARA-CK1α-His

    Plasmid

    #154074

    Purpose

    Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    CSNK1A1 (CSNK1A1 Human)
    

    Use

    Tags

    GST and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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