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Showing: 3801 - 3850 of 49475 results

  1. pMT449

    Plasmid
    #139389
    Purpose
    Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Bile Acid inducible Promoter
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139389-plasmid-map-sequence-id-313839

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  2. pMT455

    Plasmid
    #139392
    Purpose
    aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate), AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    aTC and BA inducible promoters
    Available since
    April 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    139392-plasmid-map-sequence-id-313930

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  9. mCherry-OptoPB

    Plasmid
    #113935
    Purpose
    Express a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactions
    Depositor
    Yubin Zhou
    Article
    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi
    Insert
    OptoPB
    Use
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    113935-plasmid-map-sequence-id-367689

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  12. AlkB-D135T

    Plasmid
    #160433
    Purpose
    A plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to Thr
    Depositor
    Tao Pan
    Article
    Wang et al Nucleic Acids Res. 2021 Mar 18;49(5):e30
    Insert
    E. coli AlkB
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Changed Aspartic acid 135 to Threonine; deleted t…
    Promoter
    T7
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    160433-plasmid-map-sequence-id-318950

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  14. GFP-LiMETER-v2

    Plasmid
    #113934
    Purpose
    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP
    Depositor
    Yubin Zhou
    Article
    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi
    Insert
    LOV2 and Stim1 polybasic domain (PB)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113934-plasmid-map-sequence-id-225307

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  17. pMito-MtrxRFP2

    Plasmid
    #172324
    Purpose
    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)
    Depositor
    Huiwang Ai
    Article
    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.
    Insert
    mitochondrial expression of human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    Use
    Tags
    His-tag and Mitochondria localization sequence
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, SP6
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172324-plasmid-map-sequence-id-431104

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  25. pYPQCas12j2

    Plasmid
    #189780
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoter
    Depositor
    Yiping Qi
    Article
    Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1
    Insert
    Cas12j2
    Use
    CRISPR; Gateway compatible cas12j2 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    189780-plasmid-map-sequence-id-375101

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  39. IBMc071

    Plasmid
    #161943
    Purpose
    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmid
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161943-plasmid-map-sequence-id-327544

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  42. IBMc097

    Plasmid
    #161945
    Purpose
    Contains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherry
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    161945-plasmid-map-sequence-id-326342

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  43. pMT469

    Plasmid
    #139395
    Purpose
    Nanoluc placed on Output 1 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs, AmpR, ErmR
    Depositor
    Christopher Voigt
    Article
    Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.
    Insert
    Nanoluc placed on Output 1 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    aTC and BA inducible promoters
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    139395-plasmid-map-sequence-id-313937

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Showing: 3801 - 3850 of 49475 results