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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1521 - 1540 of 129165 results

  1. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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  2. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Article
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available Since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    53261-plasmid-map-sequence-id-261354

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  10. pAAV.GfaABC1D.PI.Lck-GFP.SV40 (AAV5)

    Viral Prep
    #105598-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV.GfaABC1D.PI.Lck-GFP.SV40 (#105598). In addition to the viral particles, you will also receive purified pAAV.GfaABC1D.PI.Lck-GFP.SV40 plasmid DNA. GfaABC1D-driven GFP expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Baljit Khakh
    Article
    Shigetomi et al J Gen Physiol. 2013 Apr 15.
    Promoter
    GfaABC1D
    Tags
    GFP
    Available Since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pRSETB/mStayGold

    Plasmid
    #212017
    Purpose
    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Type
    Empty backbone
    Tags
    6xHis, T7 tag, Xpress tag, mStayGold
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212017-plasmid-map-sequence-id-419228

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  16. pRSETB/mStayGold

    Plasmid
    #212017
    Purpose
    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Type
    Empty backbone
    Tags
    6xHis, T7 tag, Xpress tag, mStayGold
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212017-plasmid-map-sequence-id-419228

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Showing: 1521 - 1540 of 129165 results