We narrowed to 2,453 results for: 683

1. pLenti-eGFP-dSaCas9-blast

   Plasmid

   #191653

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   eGFP-T2A-dSaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR4-huNFATc1nuc

   Plasmid

   #23988

   Depositor

   Jerry Crabtree

   Article

   Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

   Insert

   NFATc1 (NFATC1 Human)
   

   Use

   Cloning vector

   Tags

   EE tag

   Expression

   Mutation

   S172A S175A S176A S178A S179A S181A S184A S187A S…

   Promoter

   Available Since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEBB COMMD1 Flag

   Plasmid

   #74893

   Purpose

   Express COMMD1 in mammalian cells

   Depositor

   Ezra Burstein, Colin Duckett

   Article

   Burstein et al J Biol Chem. 2005 Jun 10;280(23):22222-3

   Insert

   COMMD1 (COMMD1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha promoter

   Available Since

   June 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNAIIIB cdc42 QL

   Plasmid

   #61636

   Purpose

   mammalian expression of cdc42 constitutively active mutant

   Depositor

   JS Gutkind

   Article

   Coso et al Cell. 1995 Jun 30;81(7):1137-46.

   Insert

   Cdc42 (CDC42 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q61L, constitutively active

   Promoter

   CMV

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pFlox_N-Mau2_Neo_FKBPF36V_mScarlet

   Plasmid

   #163881

   Purpose

   Acute Depletion of Mau2-mScarlet

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   Mau2 (Mau2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mApple-M1 SpastinK388R

   Plasmid

   #134463

   Purpose

   M1 SpastinK388R expression

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

   Insert

   M1 Spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lys to Arg at amino acid 388

   Promoter

   CMV

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-EGFP-NEMO-D304N

   Plasmid

   #187898

   Purpose

   Mammalian cell expression of D304N mutation in NEMO

   Depositor

   Erika Holzbaur

   Article

   Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

   Insert

   HA-EGFP-NEMO-D304N (Ikbkg Mouse)
   

   Use

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   D304N

   Promoter

   CMV

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHCMV_VSVgSS-diCR2

   Plasmid

   #207324

   Purpose

   Expression of secreted diCR2-10his competitor protein which can inhibit VSV-G mediated infection

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   diCR2 (LDLR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   fragment of LDL-R dimerized

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-dSaCas9-KRAB-blast

   Plasmid

   #191654

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain for gene silencing and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-TetO-Dppa2

    Plasmid

    #40799

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

    Insert

    Dppa2 (Dppa2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal CMV + TetO

    Available Since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pDY118A

    Plasmid

    #182958

    Purpose

    pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.

    Depositor

    Shelley Copley

    Article

    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12

    Inserts

    Cas9
    Gam, Beta, Exo
    I-scel
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMx/ATP11C-HA

    Plasmid

    #209237

    Purpose

    Mammalian expression of ATP11C

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP11C (ATP11C Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. sq5

    Plasmid

    #24499

    Depositor

    Bruce Conklin

    Article

    Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

    Insert

    Gs alpha (Gnas Rat)
    

    Use

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    This is Gs alpha with the C-terminal amino acids …

    Promoter

    Available Since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pJT82_GalL_nCDA1Δ198-BE3

    Plasmid

    #145046

    Purpose

    Expresses nCDA1Δ198-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ198-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-198AA); spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCerulean3-Golgi-7

    Plasmid

    #55420

    Purpose

    Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available Since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pJT25_GalL_BE3

    Plasmid

    #143736

    Purpose

    Expresses BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEAK-cKal7

    Plasmid

    #145801

    Purpose

    expresses Kalirin-7 with the c-front in mammalian cells

    Depositor

    Betty Eipper, Richard Mains

    Article

    Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

    Insert

    cKalirin-7 (Kalrn Rat)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    EIF2a

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-BBS4

    Plasmid

    #218713

    Purpose

    Expresses N-terminally EGFP-tagged BBS4 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS4 (BBS4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    I354T (t1110c)

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. mApple-M11-92

    Plasmid

    #134466

    Purpose

    M11-92 expression

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

    Insert

    M1 Spastin Amino Acids 1-92 (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M1 Spastin Amino Acids 1-92

    Promoter

    CMV

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ-dzCas9-NG-Act3.0

    Plasmid

    #158415

    Purpose

    CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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