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R620-M07-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 E484K

Plasmid

#170193

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

E484K

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M08-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP E484K

Plasmid

#170194

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

3C-His8-SBP

Expression

Mammalian

Mutation

E484K

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M03-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 N501Y

Plasmid

#170189

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.7 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

N501Y

Promoter

CMV51

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

Plasmid

#229814

Purpose

A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-EGFP-stop-lox

Use

Cre/Lox; Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP5G

Plasmid

#31788

Purpose

a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

Depositor

Douglas Kim, Loren Looger, GENIE Project

Article

Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

Insert

GCaMP5G

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pME-Actin-Vhh-sfGFP-P2A-1xHA-tdiRFP-caax (JDW 1311)

Plasmid

#224497

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and tdiRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP-P2A-HA-tdiRFP-caax

Use

Gateway cloning

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3

Plasmid

#159700

Purpose

E. coli protein expression

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Avi-Hs.LGALS3 (LGALS3 Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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R717-X57-566: His6-MBP-tev-Hs.RAF1(52-188)

Plasmid

#159697

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-188)

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Hs.RAF1(52-188) (RAF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

Plasmid

#229810

Purpose

A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

loxp-AmCyan-stop-lox

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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R703-M72-303: CMV51p> Halotag-Hs.KRAS4b

Plasmid

#159679

Purpose

Mammalian protein expression of KRAS4b-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.KRAS4b (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R713-M88-303: CMV51p> Halotag-Hs.HRAS

Plasmid

#159681

Purpose

Mammalian protein expression of HRAS-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.HRAS (HRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R713-M91-303: CMV51p> Halotag-Hs.NRAS

Plasmid

#159682

Purpose

Mammalian protein expression of NRAS-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.NRAS (NRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)

Plasmid

#159696

Purpose

E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)

Tags

His6-Halotag-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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R713-M85-303: CMV51p> Halotag-Hs.KRAS4a

Plasmid

#159680

Purpose

Mammalian protein expression of KRAS4a-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Hs.KRAS4a (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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R717-X45-566: His6-MBP-tev-Hs.RAF1(136-188)

Plasmid

#159698

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(136-188)

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Hs.RAF1(136-188) (RAF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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R725-X11-566: His6-MBP-tev-Hs.LGALS3

Plasmid

#159699

Purpose

E. coli protein expression of His6-MBP-tev-Hs.LGALS3

Depositor

Dominic Esposito

Article

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-MBP-tev-Hs.LGALS3 (LGALS3 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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18065-M03-412

Plasmid

#225666

Purpose

Lentivirus protein expression. Control plasmid.

Depositor

Dominic Esposito

Article

Sharma et al Nat Commun. 2021 Dec 15;12(1):7300. doi:

Inserts

dsCopGFP
delCD19 (CD19 Human)

Use

Lentiviral

Tags

3xFLAG

Mutation

C-terminally truncated (aa 1-333 only)

Promoter

WPRE-SV40p and mCMVp

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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R717-X61-566: His6-MBP-tev-Hs.RAF1(52-192)

Plasmid

#159687

Purpose

E. coli protein expression of His6-MBP-tev-Hs.RAF1(52-192)

Depositor

Dominic Esposito

Article

Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

Insert

His6-MBP-tev-Hs.RAF1(52-192) (RAF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R713-M50-303: CMV51p> Halotag-HRasHVR

Plasmid

#159685

Purpose

Mammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-HRasHVR (HRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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R713-M48-303: CMV51p> Halotag-Kras4aHVR

Plasmid

#159684

Purpose

Mammalian protein expression of KRAS4a HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Kras4aHVR (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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