We narrowed to 4,435 results for: Gca

1. pHW580

   Plasmid

   #198816

   Purpose

   In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, slow kinetics

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::GCaMP7s-SL2-mKate2::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDF0428 huDisCas7-11-R1045-GGGS-R1122-U6-pro-Gluc

   Plasmid

   #186985

   Purpose

   AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of Gluc crRNA guide

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

   Insert

   cas7-11-r1045-gggs-r1122, Gluc crRNA guide
   

   Use

   AAV

   Tags

   Expression

   Mutation

   r1045-gggs-r1122 deletion

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDIV495

   Plasmid

   #177702

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ATP1A1_G4_Q118R_RUNX1_+1_ATGins_Dual_pegRNA

   Plasmid

   #173213

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +1 ATG insertion pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + RUNX1 +1 ATG insertion pegRNA
   

   Use

   CRISPR; Prime editing

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tandem U6 promoters

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMKO.1-shCdc20

   Plasmid

   #160954

   Purpose

   Cdc20 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Cdc20 shRNA (Cdc20 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV2-U6gRNA5(Rictor-g1)-PGKpuroBFP-W

   Plasmid

   #105041

   Purpose

   Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Rictor (Rictor Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV2-U6gRNA5(Rictor-g2)-PGKpuroBFP-W

   Plasmid

   #105042

   Purpose

   Lentiviral gRNA plasmid targeting mouse Rictor , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Rictor (Rictor Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. gh265

   Plasmid

   #106864

   Purpose

   expression of gRNA targeting ALG11

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   ALG11 (ALG11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NDUFAB1 C4.3 gRNA

   Plasmid

   #90793

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NDUFAB1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NDUFAB1 (Guide Designation C4.3) (NDUFAB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RPS6KL1 gRNA (BRDN0001145154)

    Plasmid

    #77952

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KL1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KL1 (RPS6KL1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PAN3 gRNA (BRDN0001146135)

    Plasmid

    #77424

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PAN3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PAN3 (PAN3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SCYL2 gRNA (BRDN0001145947)

    Plasmid

    #76050

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SCYL2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SCYL2 (SCYL2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ROR1 gRNA (BRDN0001162233)

    Plasmid

    #76044

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ROR1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ROR1 (ROR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SBK3 gRNA (BRDN0001146759)

    Plasmid

    #75899

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SBK3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SBK3 (SBK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TNIK gRNA (BRDN0001146101)

    Plasmid

    #75850

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TNIK

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TNIK (TNIK Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MKNK1 gRNA (BRDN0001148208)

    Plasmid

    #75514

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MKNK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MKNK1 (MKNK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. sgGLDC_1

    Plasmid

    #72886

    Purpose

    CRISPR-Cas9 induced gene disruption

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    sgGLDC
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pMKO.1-puro/hFOXH1 shRNA6

    Plasmid

    #59310

    Purpose

    Knockdown of human FOXH1

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    hFOXH1 shRNA (FOXH1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pGL4.23-5xE-box

    Plasmid

    #206841

    Purpose

    The plasmid contains a 312 bp DNA fragment that contained 5 canonical E-boxes (GCCACGTGCA) spaced by 50 nucleotides and cloned into pGL4.23[luc2/minP] (XhoI/HindIII).

    Depositor

    Tassula Proikas-Cezanne

    Article

    Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

    Insert

    5x E-box ((GCCACGTGCA)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p426_Cas9_gRNA-YPRCd15c

    Plasmid

    #87404

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YPRCd15c sequence AATCCGAACAACAGAGCATA in yeast chromosome 14.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting YPRCd15c
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge