We narrowed to 15,274 results for: pcDNA

1. pcDNA3 Ras-LOCKR-S

   Plasmid

   #216496

   Purpose

   FRET-based reporter for Ras activity, untargeted

   Depositor

   David Baker

   Article

   Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

   Insert

   Ras-LOCKR-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-RIα-EGFP

   Plasmid

   #181840

   Purpose

   GFP-tagged PKA RIα subunit for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-EGFP (PRKAR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-PKA-SPARK

   Plasmid

   #106920

   Purpose

   Express PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   PKA-SPARK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-SypHluorin 4x (S4x)

   Plasmid

   #37005

   Depositor

   Yongling Zhu, Stephen Heinemann

   Article

   Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

   Insert

   SypHluorin 4x (Syp Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Four copies of pHluorin inserted at site 184-185

   Promoter

   CMV

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-endo-Nb80

   Plasmid

   #205518

   Purpose

   Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-Nb80
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcdna3 flag - ULK1 wt

   Plasmid

   #27636

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse ULK1 (Ulk1 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Initial M from ULK1 removed.

   Promoter

   Available since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-MS2-HA-HshnRNPM_Z

   Plasmid

   #148126

   Purpose

   Mammalian Expression of HshnRNPM

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPM (HNRNPM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   two silent mutations compared to the sequenceof t…

   Promoter

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-RIIα-Dronpa

   Plasmid

   #182331

   Purpose

   PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   RIIalpha-Dronpa (PRKAR2A Human)
   

   Use

   Tags

   Full-length human PKA type 2alpha regulatory subu…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3_PKA-RIIα-sREACh

   Plasmid

   #168489

   Purpose

   Mammalian expression of mouse PKA regulatory subunit IIα C-terminally tagged by sREACh.

   Depositor

   Haining Zhong

   Article

   Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

   Insert

   PKA regulatory subunit IIα C-terminally tagged by sREACh
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-RLuc8.6-535

    Plasmid

    #87125

    Purpose

    Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

    Depositor

    Sanjiv Sam Gambhir

    Article

    Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

    Insert

    Renilla Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-TLR8-YFP

    Plasmid

    #13024

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 8 (TLR8 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-PDGFRA-V5/HIS

    Plasmid

    #201987

    Purpose

    expression of human PDGFRA receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    PDGFRA receptor tyrosine kinase (PDGFRA Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-FLAG-mTET2

    Plasmid

    #89735

    Purpose

    Expresses wildtype mouse TET2 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    TET2 (Tet2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4-Vps34-Flag

    Plasmid

    #24398

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Vps34 (PIK3C3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-PDGFRB-V5/HIS

    Plasmid

    #201988

    Purpose

    expression of human PDGFRB receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    PDGFRB receptor tyrosine kinase (PDGFRB Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO HSPA8

    Plasmid

    #19460

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA8 (HSPA8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Dub3 mouse

    Plasmid

    #72682

    Purpose

    mammalian expression of mouse Dub3

    Depositor

    Domenico Maiorano

    Article

    van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

    Insert

    Dub3 (Usp17le Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-Antares2 c-myc

    Plasmid

    #100027

    Purpose

    A BRET based CyOFP-teLuc-CyOFP fusion to further red-shift bioluminescence

    Depositor

    Huiwang Ai

    Article

    Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

    Insert

    Antares2
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-AKAP79-AKAR4

    Plasmid

    #181957

    Purpose

    Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

    Depositor

    Jin Zhang

    Article

    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

    Insert

    AKAP79-AKAR4 (AKAP5 Human)
    

    Use

    Tags

    AKAP79, Cerulean, HIS tag, and cpVenus(E172)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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