Addgene

We narrowed to 19,679 results for: ACE

Showing: 3081 - 3120 of 19679 results

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pICH42760

Plasmid

#50257

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, spm (Z. mays)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45180

Plasmid

#50260

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, RbcS3B (AT5g38410, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45131

Plasmid

#50262

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, LHB1B2 (AT2g34420, A. thaliana)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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MSCV-PM mir shHoxB9 a

Plasmid

#27021

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

mir HoxB9 2A

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pILGFP4G

Plasmid

#104502

Purpose

Used to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

ScPGM2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pILGFP4K

Plasmid

#104506

Purpose

Used to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SpGAL1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pILGFP4O

Plasmid

#104510

Purpose

Used to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SpdGAL2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pILGFP4P

Plasmid

#104511

Purpose

Used to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SptGAL2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pHES936

Plasmid

#89199

Purpose

Expresses DIG1 under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

RST1 (DIG1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pHES938

Plasmid

#89200

Purpose

Expresses MSG5 under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

MSG 5 (MSG5 Budding Yeast)

Use

Yeast integration

Tags

Expression

Mutation

Promoter

GAL1

Available since

Availability

Academic Institutions and Nonprofits only

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pHES939

Plasmid

#89201

Purpose

Expresses GPA1 under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

GPA1 (GPA1 Budding Yeast)

Use

Yeast integration

Tags

Expression

Mutation

Promoter

GAl1

Available since

Availability

Academic Institutions and Nonprofits only

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pHES940

Plasmid

#89202

Purpose

Expresses STE4 under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

STE4 (STE4 Budding Yeast)

Use

Yeast integration

Tags

Expression

Mutation

Promoter

GAl1

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1 human PP2A Aα full length

Plasmid

#132634

Purpose

expresses human PP2A Aα full length in E. coli

Depositor

Wenqing Xu

Article

Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

Insert

PP2A Aα (PPP2R1A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human PP2A B56α full length

Plasmid

#132635

Purpose

expresses human PP2A B56α full length in E. coli

Depositor

Wenqing Xu

Article

Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

Insert

PP2A B56α

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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ER-RpHLuorin2

Plasmid

#171717

Purpose

Luminal ER expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 targeted to ER

Use

Tags

KDEL ER-retention signal and Signal sequence of h…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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GPI-RpHLuorin2

Plasmid

#171721

Purpose

GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to GPI anchor

Use

Tags

ratiometric pHluorin2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-AAVS1

Plasmid

#227272

Purpose

Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting AAVS1 (AAVS1 Synthetic)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAD-LyseR

Plasmid

#99244

Purpose

For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

Depositor

Jeff Hasty

Article

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Insert

phage lambda gene R (R phage lambda)

Use

Tags

Expression

Bacterial

Mutation

Promoter

beta-lactamase promoter

Available since

Oct. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pACS129

Plasmid

#204594

Purpose

Histone H3.1-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.1 (H3C3 Human)

Use

Lentiviral

Tags

human histone H3.1-V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ATeam1.03-nD/nA/pcDNA3

Plasmid

#51958

Purpose

mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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pCpf1b-sp

Plasmid

#122188

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCpf1b

Plasmid

#122187

Purpose

Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCpf1-sp

Plasmid

#122186

Purpose

Bacterial genome editing plasmid with spectinomycin resistance marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-C-mScarlet-Zeo

Plasmid

#207733

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pAS_sfGPF 150TAG

Plasmid

#154765

Purpose

plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPYL-ABI

Plasmid

#233650

Purpose

Cellular colocalization assay (PYL/ABI) for the CIP OP (opabactin) or the CIP ABA (abscisic acid)

Depositor

Richard Wombacher

Article

Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

Insert

TOMM20-mCherry-PYL-P2A-eGFP-ABI

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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LgBiT-ST

Plasmid

#207633

Purpose

A plasmid encoding LgBiT fused to SpyTag

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

LgBiT-SpyTag

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-C-mStayGold-Zeo

Plasmid

#227255

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pEutRwc

Plasmid

#216334

Purpose

An IPTG-inducible promoter controlling expression of eutR; PEutS, extended controlling expression of sfgfp

Depositor

Mark Styczynski

Article

McNerney et al ACS Synth Biol. 2020 Feb 21;9(2):402-411

Inserts

EutR
Superfolder green fluorescent protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Green cGull

Plasmid

#86867

Purpose

Green fluorescent probe for cGMP

Depositor

Tetsuya Kitaguchi

Article

Matsuda et al ACS Sens. 2017 Jan 27;2(1):46-51. doi: 1

Insert

Green cGull

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-N-Zeo-mStayGold

Plasmid

#227252

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mStayGold cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-N-Blast-mScarlet

Plasmid

#207678

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mScarlet cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
dCas9-5xGCN4-P2A-BFP

Plasmid

#184438

Purpose

dCas9 fused with 5 copies of GCN4 epitope

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

dCas9-5xGCN4

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-N-Blast-ultraID

Plasmid

#207682

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
lenti-gRNA

Plasmid

#226867

Purpose

Lentiviral expression of Sp-gRNA with BlpI and BstXI restriction sites for gRNA spacer cloning. Also expresses BFP.

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

Lenti Sp-gRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mU6

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-hsRPA70 (MSW#473)

Plasmid

#208067

Purpose

Expression of GFP-tagged hsRPA1 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA1 (RPA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1α-Cre-2A-Puro

Plasmid

#207743

Purpose

Expression of the Cre recombinase linked to a puro selectable marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-2A-Puro

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Blast-3xFLAG

Plasmid

#207684

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-C-3xHA-Blast

Plasmid

#207718

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Blast-V5

Plasmid

#207686

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

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