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Showing: 1541 - 1560 of 1801 results

  1. XLone-Puro NFIL3 P2A eGFP NLS

    Plasmid
    #140026
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Synthetic, Human)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140026-plasmid-map-sequence-id-275838

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  2. DN2-Gli3 in pECE

    Plasmid
    #121971
    Purpose
    medium Gli3 transcriptional activator
    Depositor
    James Briscoe
    Insert
    DN2Gli3 (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc
    Expression
    Mammalian
    Mutation
    N terminus deletion (nt1-1401)
    Promoter
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121971-plasmid-map-sequence-id-241250

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  4. pIND4-RC-C2-M210TAG+M252V-oCNF

    Plasmid
    #222995
    Purpose
    Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for ortho-phenylalanine variants
    Depositor
    Steven Boxer
    Inserts
    puhA
    pufL
    pufM
    Phe variants PylRs
    Mm Pyl tRNA for oF incorporation
    Use
    Tags
    6xHis
    Expression
    Mutation
    changed Tyr M210 to amber stop codon (TAG) for am…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    222995-plasmid-map-sequence-id-444743

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  9. EcpH-TM

    Plasmid
    #85389
    Purpose
    Expresses ecliptic pHluorin–ICAM-1 (intercellular adhesion molecule 1) in mammalian cells. Incorporates into viral membrane to label pseudoviruses
    Depositor
    Gregory Melikyan
    Article
    Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do
    Insert
    ecliptic pHluorin (EcpH) fused to N-terminus of human ICAM-1 transmembrane domain
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    85389-plasmid-map-sequence-id-169209

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  10. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid
    #37184
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Use
    Tags
    FokI nuclease
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    37184-plasmid-map-sequence-id-46727

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  11. XLone-BSD SARS-CoV2 N P2A mCherry

    Plasmid
    #154398
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154398-plasmid-map-sequence-id-304591

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  14. pGEX6P1 pS43 METTL3

    Plasmid
    #200732
    Purpose
    Bacterial expression of GST-Tagged codon optimized human pS43 METTL3
    Depositor
    Claudio Alarcón
    Insert
    pS43 METTL3 (METTL3 Human)
    Use
    Tags
    GST from backbone plasmid
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli, UAG co…
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200732-plasmid-map-sequence-id-401809

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  15. pRSF-G1(CysCNP)RS

    Plasmid
    #225054
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-cysteine (CysCNPym) into proteins in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Abdelkader et al Chem 102385
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    225054-plasmid-map-sequence-id-446766

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  17. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Article
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Promoter
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31186-plasmid-map-sequence-id-334781

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Showing: 1541 - 1560 of 1801 results